average mol wt 30,000-70,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

别名: PDL

• CAS号 27964-99-4

• 线性分子式 D-Lys-(D-Lys)_n-D-Lys · xHBr

•  MDL number MFCD00131934

•  PubChem Substance ID 24898835
  
  

  性质

  Related Categories	Amino Acids, Attachment Factors, Attachments Factors, Core Bioreagents, Life Science Reagents for Cell Culture, Polyamino Acids, Polylysine ａnd Polyornithine Attachment Factors, Research Essentials, 生化试剂, 细胞培养, 试剂和添加剂 更多...
  InChI Key	MEXAGTSTSPYCEP-UHFFFAOYSA-N
  sterility	γ-irradiated
  product line	BioReagent
  form	lyophilized powder
  mol wt	average mol wt 30,000-70,000
  suitability	suitable for cell culture
  storage temp.	−20°C

  产品描述

  Components

  分子量 >30,000 的聚赖氨酸适用于促进细胞粘附到固体基质。

  Application

  Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers ａnd cause an excessive uptake of L-lysine.
  
  This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm². Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

  Biochem/physiol Actions

  This product is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane ａnd the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.

  Caution

  无菌溶液可在2-8℃条件下稳定保存2年。脱水保存在-20°C条件下。

  Preparation Note

  分子量基于粘度。