lyophilized powder, suitable for cell culture

别名: 弹性蛋白酶 来源于猪胰腺, 胰酞酶 E

• CAS号 39445-21-1

•  Enzyme Commission (EC) Number 3.4.21.36  ( BRENDA | IUBMB )

•  EINECS 编号 254-453-6

•  MDL number MFCD00130998
  
  
  
  
  
  
  
  

  性质

  Related Categories	3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Cell Dissociation, Cell Dissociation (Cell Culture Tested), Cell Dissociation Enzymes, Cell Dissociation ａnd Cell Lysis, Cell Signaling Enzymes, Elastase, pancreatic, Enzyme Class Index, MMP Enzymes, Matrix Metalloproteinases Products, Proteolytic Enzymes, Proteolytic Enzymes ａnd Substrates, Selective Proteolytic Enzymes, 生化试剂, 细胞培养, 试剂和添加剂, 酶、抑制剂和底物 更多...
  form	lyophilized powder
  activity	≥4 units/mg protein
  suitability	suitable for
  	suitable for cell culture
  foreign activity	Trypsin ≤50 BAEE units/mg protein
  storage temp.	−20°C

  产品描述

  Biochem/physiol Actions

  Elastase hydrolyses elastin, the specific protein of elastic fibers, ａnd digests hemoglobin, casein ａnd fibrin.

  Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, ａnd Leu. The enzyme also hydrolyzes amides ａnd esters such as N-Benzoyl-L-alanine methyl ester.^[4][5]

  包装

  1, 5 mg in glass bottle

  Package size based on protein content

  20 mg in poly bottle

  Unit Definition

  在 pH 8.0 和 25°C 下，一单位每分钟可水解 1.0μmol 的 N-琥珀酰-L-Ala-Ala-Ala-对硝基苯胺。

  Application

  Elastase from porcine pancreas is used for the following applications:
  • Cell culture techniques^[1]
  • ELISA techniques^[2]
  • Used in assay for elastase inhibitory activity^[3]
   

  General description

  Elastase is a single polypeptide chain of 240 amino acid residues ａnd contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.^[4]