powder, ≥250 units/mg solid

别名: 胃液素 来源于猪胃, 胃蛋白酶 A

• CAS号 9001-75-6

•  Enzyme Commission (EC) Number 3.4.23.1  ( BRENDA | IUBMB )

•  EINECS 编号 232-629-3

•  MDL number MFCD00081840

•  eCl@ss 42010127
  
  
  

  性质

  Related Categories	3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Clinical Chemistry, Core Bioreagents, Core Bioreagents Enzymes, Enzyme Class Index, Enzymes for Diagnostic Kit Manufacturing, Pepsin, Proteases, Proteases & Protein Sequencing, Proteolytic Enzymes, Proteolytic Enzymes ａnd Substrates, Research Essentials, Selective Proteolytic Enzymes, 生化试剂, 酶、抑制剂和底物 更多...
  form	powder
  mol wt	mol wt 35 kDa
  Featured Industry	Diagnostic Assay Manufacturing
  shipped in	wet ice
  storage temp.	2-8°C
  Gene Information	pig ... LOC396892(396892)

  产品描述

  Analysis Note

  *佳 pH 值为 2-4。在 4M 脲和 3M 盐酸胍中具有活性。在 60°C 下能稳定存在。在 pH 8.0-8.5 下可对胃蛋白酶进行不可逆地灭活。

  Application

  用于产生抗体的 F(ab′)₂ 片段。

  Pepsin is a peptidase used to digest proteins ａnd is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice. Product P7000 is provided as a powder ａnd has been used to treat epithelial cells from a single feline mammary carcinoma^[1].

  The enzyme from Sigma has been used to simulate in vitro gastric digestion of cooked cod.^[4] It has been used to simulate in vitro gastric digestion of cocoa mass ａnd supplemented dietary fiber.^[3] It has also been used to increase the fraction of extractable soluble collagen ａnd to lower the immunogenicity of the resulting collagen from bovine dermal tissue.^[2]

  Biochem/physiol Actions

  优先裂解：P1 和 P1′ 位的疏水性和芳族残基。降解胰岛素 β 链中的 Phe-Val、Gln-His、Glu-Ala、Ala-Leu、Leu-Tyr、Tyr-Leu、Gly-Phe、Phe-Phe 和 Phe-Tyr 键

  Pepsin hydrolyzes peptide bonds, not amide or ester linkages. Pepsin cleaves peptides with an aromatic acid on either side of the peptide bond. Sulfur-containing amino acids increase susceptibility to hydrolysis when they are close to the peptide bond. Pepsin preferentially cleaves at the carboxyl side of phenylalanine ａnd leucine ａnd at the carboxyl side of glutamic acid residues. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe ａnd Phe-Tyr bonds in the β chain of insulin
  Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages..

  The enzyme does not cleave at valine, alanine, or glycine linkages.^[6] Z-L-tyrosyl-L-phenylalanine, Z-L-glutamyl-L-tyrosine, ａnd Z-L-methionyl-L-tyrosine may be used as substrates for pepsin digestion.^[5][6] Pepsin is inhibited by several phenylalanine-containing peptides.^[7]

  Other Notes

  View more information on pepsin at www.sigma-aldrich.com/enzymeexplorer.

  包装

  1 kg in poly bottle

  25, 100 g in poly bottle

  Unit Definition

  One unit will produce a ΔA₂₈₀ of 0.001 per min at pH2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16ml. Light path = 1cm.)