lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280, 18.3)

别名: IIa 因子

• CAS号 9002-04-4

•  Enzyme Commission (EC) Number 3.4.21.5  ( BRENDA | IUBMB )

•  EINECS 编号 232-648-7

•  MDL number MFCD00082072
  
  

  性质

  Related Categories	3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Coagulation Proteins ａnd Reagents, Core Bioreagents, Core Bioreagents Enzymes, Enzyme Class Index, Plasma & Blood Proteins, Plasma, Blood, ａnd Related Proteins ａnd Reagents, Proteases, Proteases & Protein Sequencing, Proteases for Recombinant Protein Fusion Tag Cleavage, Proteolytic Enzymes, Proteolytic Enzymes ａnd Substrates, Research Essentials, Selective Proteolytic Enzymes, Serum Proteins ａnd Related Enzymes, Serum Related Enzymes, Thrombin, 生化试剂, 蛋白质及衍生物, 酶、抑制剂和底物 更多...
  form	lyophilized powder
  impurities	HIV ａnd HBsAg, source material tested negative
  storage temp.	−20°C
  Gene Information	human ... F2(2147)

  产品描述

  Analysis Note

  NIH 试验步骤基于 Biggs 方法的改进，使用 0.2mL 稀释血浆（与生理盐水 1:1 混合，作为底物）和 0.1mL Biggs 凝血酶样品（稳定于 1% 缓冲白蛋白溶液中）。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。

  Biochem/physiol Actions

  丝氨酸蛋白酶，可选择性裂解血纤维蛋白原的 Arg-Gly 键，形成血纤维蛋白和血纤维蛋白肽 A 和 B。

  General description

  Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant ａnd anticoagulant effects.

  Other Notes

  查看更多关于 凝血酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer。

  Unit Definition

  Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard

  Physical form

  Lyophilized from saline sodium citrate buffer, pH 6.5

  Application

  Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to investigate the protein C pathway in intestinal barrier function. ^[1]