for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

别名: RNase A, 核糖核酸 3′-嘧啶寡核苷酸水解酶, 核糖核酸酶 I, 胰核糖核酸酶

• CAS号 9001-99-4

•  Enzyme Commission (EC) Number 3.1.27.5  ( BRENDA | IUBMB )

•  EINECS 编号 232-646-6

•  MDL number MFCD00132181
  
  

  性质

  Related Categories	3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Core Bioreagents, Enzyme Class Index, Life Science Reagents for Cloning, Life Science Reagents for Cloning Enzymes, Modifying Enzymes, Molecular Biology Tested, Plant Nucleic Acid Purification Enzymes, Protein ａnd Nucleic Acid Isolation, Research Essentials, 分子生物学, 分子生物学酶, 植物分子生物学, 植物核酸纯化, 植物生物技术, 生化试剂, 酶、抑制剂和底物 更多...
  grade	for molecular biology
  form	lyophilized
  mol wt	mol wt 13.7 kDa
  	mol wt ~13,700
  foreign activity	Exonuclease ａnd endonuclease, detected
  	NICKase ａnd DNase, detected
  storage temp.	−20°C

  产品描述

  Analysis Note

  通过 E1%/280 确定的蛋白质。

  Features ａnd Benefits

  • RNase protection assays
  • Remove unspecifically bound RNA
  • Analysis of RNA sequences
  • Hydrolyze RNA contained in protein samples
  • Purification of DNA
   

  General description

  RNase A is an endoribonuclease that attacks at the 3’OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp ａnd A-pG. The highest activity is exhibited with single stranded RNA.

  Other Notes

  A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 μg/mL.
  
  Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary ａnd may cause precipitation of RNase ａnd possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.

  Activators of RNase A include potassium ａnd sodium salts. RNase A can be inhibited by alkylation of His12 or His119.

  Application

  Ribonuclease A is used to remove RNA from DNA plasmid preparations ａnd protein samples. It is also used in RNA sequence analysis ａnd protection assays.