上海劲马生物销售各种ELISA试剂盒，有RD，RB原装，标本齐全，提供代测。如有疑问，可与上海劲马生物销售或技术联系 负责人：王经理。
产品名称：小鼠T细胞活化连接蛋白（LAT）ELISA试剂盒
英文名称：Mouse Linker for activation of T cell,LAT ELISA KIT
产品规格：96人份/48人份
各种种属：人、大小鼠、豚鼠、兔子、猪、犬、牛、羊…
标本齐全：血清、血浆、细胞培养上清液、组织匀浆、组织液、关节液、胸腔溢液等…
保存条件：2-8℃
联系电话：021-6051 7348,138 1714 0470
提供ELISA代做实验  在我司购买试剂盒提供免费代测
Assay procedure

1.       Dilute ａnd add sample:Dilute Original density Standard as follow table:

2.add sample：Set blank wells separately （blank comparison wells don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same）. testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl （sample final dilution is 5-fold）, add sample to wells , don’t touch the well wall as far as possible, and Gently mix.

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold（or 20-fold） wash solution diluted 30-fold （or 20-fold） with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discardLiquid, dry by swing, add washing buffer to every well, still for 30s then drain,repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction（the blue color change to yellow color）.

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

“小鼠T细胞活化连接蛋白（LAT）ELISA试剂盒”含税含运费，提供代测，中英文双版说明书，品牌美国RD、RB、IBL、Omega，质量，详情请来电向我司索取信息。