大鼠α1微球蛋白（α1-MG）ELISA试剂盒 96T/48T 均备现货！
上海劲马生物科技有限公司是国内主要供应商，公司代理美国原装R&D RB BD等不同品牌价格档次的ELISA试剂盒。数万种抗体产品等, 品种多，质量好，灵敏度高，价格实惠，并且还提供免费代检测服务。本公司的更多Elisa试剂盒，请点击公司网址：http://www.jmbiosh.com
Assay procedure
1. Dilute ａnd add sample:Dilute Original density Standard as follow table:
160ng/L 5 Standard 150μl Original density Standard+150μl Standard diluent
80ng/L 4 Standard 150μl 5 Standard+150μl Standard diluent
40ng/L 3 Standard 150μl 4 Standard+150μl Standard diluent
20ng/L 2 Standard 150μl 3 Standard +150μl Standard diluent
10ng/L 1 Standard 150μl 2 Standard +150μl Standard diluent
2.add sample：Set blank wells separately （blank comparison wells don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same）. testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl （sample final dilution is 5-fold）, add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold（or 20-fold） wash solution diluted 30-fold （or 20-fold） with distilled water ａnd reserve.
5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.
7.incubate：Operation with 3.
8.washing：Operation with 5.
9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction（the blue color change to yellow color）.
11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

“大鼠α1微球蛋白（α1-MG）ELISA试剂盒 ”可检测样本：血液标本，组织液标本，尿液标本，粪便标本，脑脊液标本，胸腹水标本等（具体详细情况请咨询）。