l      When not in use, kit components should be stored refrigerated or frozen as indicated on vials or bottles. All reagents should be warmed to room temperature before use. Lyophilized standards should be discarded after use.

2     Once the desired number of strips has been removed, immediately reseal the bag to protect the remaining strips from edterioration.

3     Cover or cap all reagents when not in use.

4     Do not mis or interchange reagents between different lots.

5      Do not use reagents beyond the expiration date of the kit .

6       Use a clean disposable plastic pipette tip for each reagent, standard, or specimen addition in order to avoid cross-contamination, for the dispensing of H2SO4 ａnd substrate solution, avoid pipettes with metal parts.

7      Use a clean plastic container to prepare the washing solution.

8      Thoroughly mix the reagents ａnd samples before use by agitation or swirling.

9      All residual washing liquid must be drained from the wells by efficient aspiration or by decantation followed by tapping the plate forcefully on absorbent paper. Never ｉnsert absorbent paper directly into the wells.

10     The TMB solution is light sensitive. Avoid prolonged exposure to light, also, avoid contact of the TMB solution with metal to prevent colour development. Warning TMB is toxic avoid direct contact with hands. Dispose off properly. If a dark blue colour develops within a few minutes after preparation, this indicates that the TMB solution has been contaminated ａnd must be discarede. Read absorbances within 1 hour after completion of the assay.

11      When pipetting reagents, maintain a consistent order of addition from well-to-well. This will ensure equal incubation times for all wells.

12       Respect incubation times described in the assay procedure.