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大鼠免疫球蛋白E(IgE)ELISA Kit
Rat Immunoglobulin E,IgE ELISA Kit
试剂盒组成:
1、30倍浓缩洗涤液 20ml×1瓶 7 终止液 6ml×1瓶
2、酶标试剂 6ml×1瓶 8 标准品(240 ng/L) 0.5ml×1瓶
3、酶标包被板 12孔×8条 9 标准品稀释液 1.5ml×1瓶
大鼠免疫球蛋白E(IgE)ELISA Kit 说明:
操作步骤:
1.洗涤:小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干。
2.加酶:每孔加入酶标试剂50μ小鼠吡啶交联物ELISA试剂盒,空白孔除外。
3.温育:
4.洗涤:
5.显色:每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.
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