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大鼠组织蛋白酶K(cath-K)ELISA Kit
Rat Cathepsin K,cath-K ELISA Kit
操作步骤:
参照说明书(具体说明书请发邮件)
灵敏度:
具体请查看说明书.
检测范围(RANGN):
具体请查看说明书.
参考值:
大鼠组织蛋白酶K(cath-K)ELISA Kit 说明:
组织标本:
切割标本后,称取重量。加入一定量的 PBS ,缓冲液中可加入 1 μ g/L 蛋白酶抑制剂或 50U/ml 的 Aprotinin ( 抑肽酶)。用手工或匀浆器将标本匀 浆充分。离心 20 分钟左右( 2000-3000 转 / 分)。仔细收集上清置于 -20 度或 - 70 度保存,如有必要,可以将样品浓缩干燥。分装后一份待检测,其余冷冻备用。
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