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兔子神经特异性烯醇化酶(NSE)ELISA试剂盒
点击次数:329发布时间:2012/1/10 12:10:36
更新日期:2022/8/20 18:54:33
所 在 地:中国大陆
产品型号:
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详细内容
兔子神经特异性烯醇化酶(NSE)ELISA试剂盒
实验原理
用纯化的抗体包被微孔板,制成固相载体,往包被抗IL-9抗体的微孔中依次加入标本或标准品、生物素化的抗IL-9抗体、HRP标记的亲和素,经过洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。颜色的深浅和样品中的IL-9呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样品浓度。。
兔子神经特异性烯醇化酶(NSE)ELISA试剂盒
需要而未提供的试剂和器材
1. 标准规格酶标仪
2. 高速离心机
3. 电热恒温培养箱
4. 干净的试管和Eppendof管
5. 系列可调节移液器及吸头,一次检测样品较多时,用多通道移液器
6. 蒸馏水,容量瓶等
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