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兔环磷酸腺苷(cAMP)ELISA试剂盒
标本的稀释原则:
首先通过文献检索的方式了解待测样本的大致含量,确定适当的稀释倍数。只有稀释至标准曲线的范围内,检测的结果才是的。稀释的过程中,应做好详细的记录。计算浓度时,稀释了“N”倍,标本的浓度应再乘以“N”。
兔环磷酸腺苷(cAMP)ELISA试剂盒
标准品的稀释原则:
2瓶,每瓶临用前以样品稀释液稀释至1ml,盖好后静置10分钟以上,然后反复颠倒/搓动以助溶解,其浓度为500 pg/ml,做系列倍比稀释后,分别稀释500 pg/ml,250 pg/ml,125 pg/ml,62.5 pg/ml,31.2 pg/ml,15.6 pg/ml,7.8 pg/ml,样品稀释液直接作为标准浓度0 pg/ml,临用前15分钟内配制。
如配制250 pg/ml标准品:取0.5ml(不要少于0.5ml)500 pg/ml的上述标准品加入含0.5ml样品稀释液的Eppendorf管中,混匀即可,其余浓度以此类推。
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