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人α/β干扰素受体(IFN-α/βR)ELISA Kit
Human Interferon α/βReceptor,IFN-α/βR ELISA Kit
操作步骤:
1.温育:用封板膜封板后置37℃温育30分钟。
2.配液:将30倍浓缩洗涤液用蒸馏水30倍稀释后备用
3.洗涤:小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干。
4.加酶:每孔加入酶标试剂50μl,空白孔除外。
5.温育:
6.洗涤
计算:
以标准物的浓度为横坐标,OD值为纵坐标,在坐标纸上绘出标准曲线,根据样品的OD值由标准曲线查出相应的浓度;再乘以稀释倍数;或用标准物的浓度与OD值计算出标准曲线的直线回归方程式,将样品的OD值代入方程式,计算出样品浓度,再乘以稀释倍数,即为样品的实际浓算
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