【文献标题】 In utero and?lactational exposure to?BDE-47 promotes obesity development in?mouse offspring fed a?high-fat diet: impaired lipid metabolism and?intestinal dysbiosis
【作者】Dezhen Wang,Jin Yan,Miaomiao Teng,et.al
【作者单位】中国农业大学（China Agricultural University）
【文献中引用产品】
小鼠甲状腺素（T4）elisa试剂盒
【关键词】 BDE-47，Obesity， NAFLD，Gut microbiome，Metabolomics
【DOI】 doi.org/10.1007/s00204-018-2177-0
【影响因子(IF)】 2.85
【出版期刊】 《REPRODUCTIVE TOXICOLOGY》
【产品原文引用】
Blood measurements
Blood glucose levels were determined using a glucose meter (Beijing Yicheng JSP-7, China). Serum aspartate aminotransferase (AST) ａnd alanine aminotransferase (ALT) activities were measured using their respective assay kits from Nanjing Jiancheng Bioengineering Institute. Serum thyroxin (T4) levels were determined by enzyme-linked immunosorbent assay (ELISA) kits (Hengyuan Biological Technology Co., Ltd., Shanghai, China).
Glucose tolerance test
A glucose tolerance test (GTT) was performed to assess glucose homeostasis by evaluating the effectiveness of the clearance of the glucose load administrated by oral gavage. At 14?weeks, mice were orally administrated D-glucose in sterilized 0.9% NaCl after overnight fasting, ａnd glucose were measured before ａnd 15, 30, 45, 75, ａnd 105?min after glucose loading.

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