【文献标题】Effects of phoeriod on morphology ａnd function in testis ａnd epididymis of Cricetulus barabensis

【作者】Junjie Mou，Jinhui Xu，Zhe Wang，et.al

【作者单位】曲阜师范大学（Qufu Normal University）

【文献中引用产品】

仓鼠褪黑素（MT）elisa试剂盒

仓鼠促卵泡生长激素（FSH）ELISA试剂盒

仓鼠促黄体生成素（LH）ELISA试剂盒

仓鼠睾酮（T）ELISA试剂盒

【关键词】apoptosis，auhagy，Cricetulus barabensis，mitochondria，phoeriod

【DOI】10.1002/jcp.29998

【影响因子(IF)】5.20

【出版期刊】《journal of cellular physiology》

【产品原文引用】

At the end of the exposure, the animals were killed by CO2 asphyxiation at 22:00 on the final day after all of the hamsters had been in the dark for at least 2 hr (Z. Wang, Xu, Mou, Kong, Wu,et al., 2020). Blood samples were immediately collected after sacrifice ａnd stored at 4°C for 30 min, then centrifuged at 3,000 rpm for 15 min at 4°C. Serum MT, FSH, LH, ａnd fecal TTE levels were estimated using an enzyme‐linked immunosorbent assay Labsystems Multiskan MS 352 (Shanghai Hengyuan Biological Technology Co.,Ltd., H‐40277, Shanghai, China). Testis ａnd epididymis were removed ａnd weighed. The right testis or epididymis was cut into three parts;one part was immersed in paraformaldehyde‐glutaraldehyde fixative for transmission electron microscopy (TEM), while the other two parts were immersed in 4% paraformaldehyde for paraffin ａnd frozen section embedding. The left testis or epididymis was stored in a refrigerator at 80°C for subsequent western blot experiments.

完整版PDF文献请咨询在线客服或者电话联系我司业务员！

更多公司福利请关注“恒远生物”微信公众号！

原创作者：上海恒远生物技术发展有限公司