【作者】  Hao Lu, HongyuTian, Min Zhang，et al.

【作者单位】  山东农业大学

 

 

【影响因子（IF）】 4.29

 

【DOI】  https://doi.org/10.1038/s41598-020-62611-w

 

【出版期刊】《SCIENTIFIC REPORTS》

 

 

Pot experiment. 

Four treatments were carried out to verify the fertilizer efciency of CDAP:

(1) without phosphate fertilizer (P0);

(2) DAP (P1);

(3) 40% CDAP with 60% DAP (C40P1); 

(4) 60% CDAP with 40% DAP (C60P1).

Te amount of phosphate ertilizer applied to all treatments with phosphate fertilizer was 3.2 g P2O5 pot-¹. Te CDAP used in the pot experiment was WAT2 with 3% coating materials. Except for DAP ａnd CDAP, the rest of nitrogen ａnd phosphate fertilizer was supplied by common urea ａnd calcium superphosphate, respectively. Te nitrogen application amount of all treatments was 3.2 gN pot-¹. Te application rate of potassium is 1.6 g K2O pot-¹ as potassium chloride. All fertilizers were mixed evenly in 15 kg soil ａnd put into a pot 

with 10 kg sand at the bottom.

Auxin (IAA), gibberellin (GA), phosphoenolpyruvate carboxylase (PEPC), ADP-glucosepyrophosphorylase (AGPase), phosphoribosyl pyrophosphate (PRPP),adenosine monophosphate synthetase (AMPSS) and phosphoribosyl pyrophosphate aminotransferase (PRPPAT)were extracted ａnd assayed with a ELISA kit from Shanghai HengYuan Biological Technology Co. Ltd. (Shanghai, China). Soil available P content was extracted with 0.5 mol L-¹ sodium bicarbonate solution, ａnd the absorbance was measured by spectrophotometer afer molybdenum ａnd antimony. Phosphorus use efciency (PUE)=(P accumulation in P area - accumulation of P 

in blank area)/P application rate × 100%