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技术文章
恒远产品文献: 小鼠8羟基脱氧鸟苷,小鼠过氧化脂质ELISA试剂盒引用文献
点击次数:14571 发布时间:2022/2/15 10:53:22
【文献标题】Reparative effects of lycium barbarum polysaccharide on mouse ovarian injuries induced by repeated superovulation
【作者】Bing Liu,Jun-Li Wang,Xin-Mei Wang,et.al
【作者单位】北京农业大学(Beijing University of Agriculture)
【文献中引用产品】
小鼠8羟基脱氧鸟苷(8-OHdG)ELISA试剂盒
小鼠过氧化脂质(LPO)ELISA试剂盒
【关键词】Superovulatory times,Injury model,LBP,Remedy,Oxidative stress
【DOI】https://doi.org/10.1016/j.theriogenology.2020.01.048
【出版期刊】《Theriogenology》
【产品原文引用】
8-OHdG and LPO levels of the collected ovarian tissues were determined using an ELISA kit, according to the manu-facturer’s instructions (Shanghai Hengyuan Biotechnology Co., Ltd,Shanghai, China). The collected mouse ovaries were cracked with lysate. Each 10 mL henylmethane-sulfonyl (PMSF) was added in 1 mL radio immuno precipitation assay (RIPA) as lysate. Every 1 g of tissue with 9 mL lysate, then coated with mouse antibody against 8-OHdG or LPO. After incubation and washing concentrations of 8-OHdG or LPO were determined as described previously .Intra-and inter-CVs: 8-OHdG (6.3%, 8.1%), LPO (5.2%, 7.4%).
原创作者:上海恒远生物技术发展有限公司