ELISA试剂盒 ELISA KIT Elisa检测试剂盒

产品名称：人P27蛋白（P27 ）

英文名称：Human P27 protein ELISA KIT

规格：96人份/48人份

包装：进口、国产、分装、原装

人P27蛋白（P27 ）主要作用：科研

试剂盒种属：人、大小鼠、豚鼠、猪、鸡、兔、羊、猴、兔、马...

待检样本：体液，血清，血浆，细胞培养上清液，尿液，组织匀浆,心房水标本等等。

人P27蛋白（P27 ）注意事项（中文）                                         

1．试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用，酶标包被板开封后如未用完，板条应装入密封袋中保存。

2．浓洗涤液可能会有结晶析出，稀释时可在水浴中加温助溶，洗涤时不影响结果。

3．各步加样均应使用加样器，并经常校对其性，以避免试验误差。一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样。

4．请每次测定的同时做标准曲线，做复孔。如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值），请先用样品稀释液稀释一定倍数（n倍）后再测定，计算时请乘以总稀释倍数（×n×5）。

5．封板膜只限一次性使用，以避免交叉污染。

6．底物请避光保存。

7．严格按照说明书的操作进行，试验结果判定必须以酶标仪读数为准.

8．所有样品，洗涤液和各种废弃物都应按传染物处理。

9．本试剂不同批号组分不得混用。

10.如与英文说明书有异，以英文说明书为准。                          

Important notes（英文）                                               

1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .

4.if the testing material content is excessively higher （The sample OD is bigger than the first standard well ）,please dilute Sample （n-fold）, Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.The substrate evade the light preservation.

7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.Do not mix reagents with those from other lots.

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