产品展示
GST琼脂糖凝胶4FF
点击次数:96发布时间:2012/3/15 8:59:37
更新日期:2014/8/11 17:18:51
所 在 地:中国大陆
产品型号:F0063
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详细内容
GST琼脂糖凝胶4FF/谷胱甘肽琼脂糖凝胶4FF/Glutathione Sepharose 4FF
英文名称:Glutathione Sepharose 4FF
其他名称:谷胱甘肽琼脂糖凝胶4FF
级别:BR
Ligand:glutathione and 10-carbon linker arm
Ligand concentration:120-320umol glutathione/ml medium
Binding capacity:~10mg recombinant glutathione S-transferase/ml medium,GST Mr26000
Dynamic binding capacity:~11mg GST-tagged protein/ml medium Mr43000(GSTrap FF 1ml at 1 ml/min=156cm/h)
Mean particle size:90um
Bead structure:highly cross-linked 4% agarose
Maximum flow rate:450cm/h(15ml/min),using xk16/20 column with 5cm bed height,run at room temperature with aqueous buffer
Recommended flow rate:Sample loading:<100cm/h(<3ml/min using XK16/20 column);Washing and elution:100~300ml/min using XK16/20 column)
PH stability:3-12
Chemical stability:All commonly used aqueous buffers,e.g. 1M acetate pH 4.0 and 6M guanidine hydrochloride for 1 hour at room temperature
性状:含20%乙醇,底部为乳白色胶体
用途:生化研究。专门用于纯谷胱甘肽S-转移酶(GST)融合蛋白、其他的谷胱甘肽转移酶以及与谷胱甘肽有亲和作用的亲和色谱填料,一步分离就可得到很纯的目标蛋白,纯化条件温和,可蛋白的活性
保存:2~8℃