磷酸化乙酰辅酶A羧化酶抗体Anti-Phospho-Acetyl CoA Carboxylase（Ser79）的产品详细说明：

－－产品介绍：In cells, excess of metabolic fuel is converted into fatty acids in cytosol ａnd oxidized later in mitochondria to generate ATP ａnd acetyl CoA. In fatty acid synthesis, catalytic formation of malonyl CoA （precursor for long chain fatty acyl CoA, LCFA CoA） from acetyl CoA by Acetyl CoA carboxylase （ACC1） is the rate limiting step. The translocation of LCFA CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases （CPT1 ａnd CPT2） ａnd regulated by ACC2, the rate limiting step of mitochondrial fatty acid beta oxidation. Activities of ACC1 ａnd 2 are regulated by their phosphorylation by 5＇ AMP activated protein kinase （AMPK）. Diabetes deranges AMPK ｍaster switch ａnd represses the ACC1 gene expression ａnd stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. ACC1 is also known as ACC alpha is a cytosolic enzyme, enriched in liver, adipose ａnd lactating mammary tissues.ACC1 catalyzes the carboxylation of acetyl CoA to form malonyl CoA, the rate limiting step in the biogenesis of LCFA CoA. ACC1 carries three functions: biotin carboxyl carrier protein, biotin carboxylase, ａnd carboxyltransferase （catalytic activity）. Two variants of ACC1 have been described. One with 8 additional amino acids commencing at Pro 1196. The other which is 59aa shorter than the predominant fat ａnd liver isoform exist in mammals. The presence of 8 additional amino acids inhibits the in vitro phosphorylation of the Ser1200 by cAMP dependent kinase. The two ACC1 isoform are differentially regulated in a tissue specific manner ａnd under different physiological conditions. The activity of ACC1 is finely regulated by hormone dependent phosphorylation ａnd dephosphorylation.

（1mg/1ml磷酸化乙酰辅酶A羧化酶抗体Anti-Phospho-Acetyl CoA Carboxylase（Ser79），的来源为Rabbit，其相关产品：免疫组化常用试剂免疫印迹常用试剂各种标记二抗）

－－磷酸化乙酰辅酶A羧化酶抗体的参数标准：
浓度：1mg/1ml
交叉反应：hu, pig, hrs
产品类型：一抗磷酸化抗体
研究领域：细胞生物免疫学激酶和磷酸酶
蛋白分子量：predicted molecular weight: 266kDa
性状：Lyophilized or Liquid
免疫原：KLH conjugated Synthesised phosphopeptide derived from human Acetyl CoA Carboxylase around the phosphorylation site of Ser79
亚型：polyclonal
纯化方法：affinity purified by Protein A
储存液：0.01M PBS, pH 7.4 with 10 mg/ml BSA ａnd 0.1% Sodium azide
产品应用：ELISA=1:500-1000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

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