磷酸化4E结合蛋白1,2,3抗体Anti-phospho-eIF4EBP1/2/3（Thr36）的产品详细说明：

－－产品介绍：The multisubunit eukaryotic translation initiation factor （eIF） 4F recruits 40S ribosomal subunits to the 5＇ end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5＇ cap structure. Assembly of the eIF4F complex is inhibited by a family of repressor polypeptides, the eIF4E-binding proteins （4E-BPs）. 4E-BP1 （also known as PHAS-1） normally binds eIF4E, inhibiting cap-dependent translation. Hyper-phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3-kinase/Akt pathway ａnd the FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues ａnd phosphorylation by FRAP/mTOR on Threonine 37 ａnd Threonine 46 of human 4E-BP1 may prime it for sub-sequent phosphorylation at sites including Serine 65 ａnd Threonine 70. The corresponding rat residues include Threonine 36, Threonine 45, Serine 64 ａnd Threonine 69. In vitro, 4E-BP1 is also phosphorylated by ataxia telangiectasia （ATM） at human Serine 112 （rat Serine 111） in response to an increase in insulin levels.

（1mg/1ml磷酸化4E结合蛋白1,2,3抗体Anti-phospho-eIF4EBP1/2/3（Thr36），的来源为Rabbit，其相关产品：免疫组化常用试剂免疫印迹常用试剂各种标记二抗）

－－磷酸化4E结合蛋白1,2,3抗体的参数标准：
浓度：1mg/1ml
交叉反应：hu, mo, rat
产品类型：一抗
研究领域：肿瘤心血管细胞生物神经生物学信号转导细胞周期蛋白转录调节因子激酶和磷酸酶
蛋白分子量：predicted molecular weight: 20kDa
性状：Lyophilized or Liquid
免疫原：KLH conjugated synthetic peptide derived from hu phospho-eIF4EBP1/2/3（Thr36）
亚型：polyclonal
纯化方法：affinity purified by Protein A
储存液：Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用：WB=1:100-500ELISA=1:500-1000IP=1:20-100IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

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