RNA结合蛋白LIN28B抗体Anti-Lin-28B的产品详细说明：

－－产品介绍：Acts as a suppressor of microRNA （miRNA） biogenesis by specifically binding the precursor let-7 （pre-let-7）, a miRNA precursor. Acts by binding pre-let-7 ａnd recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer ａnd undergo degradation. Specifically recognizes the 5＇-GGAG-3＇ motif in the terminal loop of pre-let-7. Also recognizes ａnd binds non pre-let-7 pre-miRNAs that contain the 5＇-GGAG-3＇ motif in the terminal loop, leading to their terminal uridylation ａnd subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.Subcellular Location : Cytoplasm. Nucleus. Predominantly cytoplasmic at G1 phase, accumulates in the nucleus in S ａnd G2 phases. The frequency of nuclear localization in S ａnd in G2 phases is 60% ａnd 30%, respectively.Tissue Specificity : High expression in testis, fetal liver, placenta ａnd in hepatocellular carcinoma （HCC）. Isoform 1 is only detected in moderately ａnd poorly differentiated HCC tissues ａnd placenta （at protein level）. Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues （at protein level）.Similarity : Belongs to the lin-28 family.Contains 2 CCHC-type zinc fingers.Contains 1 CSD （cold-shock） domain.Subunit : nteracts with ZCCHC11/TUT4.

（1mg/1mlRNA结合蛋白LIN28B抗体Anti-Lin-28B，的来源为Rabbit，其相关产品：免疫组化常用试剂免疫印迹常用试剂各种标记二抗）

－－RNA结合蛋白LIN28B抗体的参数标准：
浓度：1mg/1ml
交叉反应：hu, mo, rat, chk, pig, hrs, cow, shp, Rb
产品类型：一抗
研究领域：肿瘤细胞生物免疫学表观遗传学
蛋白分子量：predicted molecular weight: 27kDa
性状：Lyophilized or Liquid
免疫原：KLH conjugated synthetic peptide derived from human Lin-28B
亚型：polyclonal
纯化方法：affinity purified by Protein A
储存液：Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用：WB=1:100-500ELISA=1:500-1000IP=1:20-100IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

上海田源专业销售RNA结合蛋白LIN28B抗体抗体产品，详细抗体目录请访问：http://www.chem17.com/st233816/erlist_675408.html