产品展示
重组膜粘连蛋白5抗体,Anti-Annexin V,一抗 价格优惠 上海现货
点击次数:152发布时间:2012/7/16 20:05:03
更新日期:2013/4/11 11:52:47
所 在 地:其它
产品型号:
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详细内容
重组膜粘连蛋白5抗体Anti-Annexin VAnchorin CI; Annexin 5; Annexin A5; Annexin V; Annexin5; AnnexinA5; AnnexinV; ANX 5; ANX A
360TIYO.COM-上海田源公司
性能参数:
中文名称:重组膜粘连蛋白5抗体
英文名称:Anti-Annexin V
别名:Anchorin CI; Annexin 5; Annexin A5; Annexin V; Annexin5; AnnexinA5; AnnexinV; ANX 5; ANX A
包装号:G320
产地:中国大陆/进口产品
商标/品牌:Santa、Abcam、MBL、BD、Cell Signaling Technology
供应商:田源试剂 TR、新试剂 NR
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产品详细描述
产品介绍:Annexins are a family of structurally related proteins that can bind specifically to cellular membranes (Cruetz, 1992). Annexin V has anticoagulant activity and is a monomer with a molecular weight of 35.8 kDa (Maurer-Fogy, 1989). It has a very high affinity for membranes containing the negatively charged phospholipid phosphatidylserine (PS). Specific binding is rapid and calcium-dependent. Its equilibrium dissociation constant is about 10-10 M in the presence of >1.5 mM Ca2+ (Andree, 1990), and specific binding is abolished if the Ca2+ is chelated by EDTA.Annexin-V是一种分子量为35~36KD的Ca2+依赖性磷脂结合蛋白,能与PS高亲和力特异性结合。将Annexin-V进行荧光素(FITC、PE)或biotin标记,以标记了的Annexin-V作为荧光探针,利用流式细胞仪或荧光显微镜可检测细胞凋亡的发生。其意义:检测早期凋亡的细胞,同时可区分活细胞和坏死细胞磷脂酰丝氨酸(Phosphatidylserine,PS)正常位于细胞膜的内侧,但在细胞凋亡的早期,PS可从细胞膜的内侧翻转到细胞膜的表面,暴露在细胞外环境中。碘化丙啶(propidine iodide,PI)是一种核酸染料,它不能透过完整的细胞膜,但在凋亡中晚期的细胞和死细胞,PI能够透过细胞膜而使细胞核红染。因此将Annexin-V与PI匹配使用,就可以将凋亡早晚期的细胞以及死细胞区分开来。重组膜粘连蛋白5抗体的质量标准:
■其它名称: | Anchorin CI; Annexin 5; Annexin A5; Annexin V; Annexin5; AnnexinA5; AnnexinV; ANX 5; ANX A |
■交叉反应: | hu |
■产品类型: | 一抗 |
■应用领域: | 细胞凋亡 |
■蛋白分子: | predicted molecular weight: 101kDa |
■浓度值: | 1mg/1ml |
■物理性状: | Lyophilized or Liquid |
■免疫原: | recombind human Annexin V |
■亚型: | polyclonal |
■纯化方法: | affinity purified by Protein A |
■保存液: | 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide |
■产品应用: | WB=1:100-500ELISA=1:500-1000IP=1:20-100IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user. |
■保存条件: | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
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重组膜粘连蛋白5抗体Anti-Annexin V的相关产品目录:
(1mg/1ml重组膜粘连蛋白5抗体Anti-Annexin V,Anchorin CI; Annexin 5; Annexin A5; Annexin V; Annexin5; AnnexinA5; AnnexinV; ANX 5; ANX A的来源为Rabbit,其相关产品:免疫组化常用试剂免疫印迹常用试剂各种标记二抗)
查询其它抗体目录请点击本公司化工仪器网链接:http://www.chem17.com/st233816/erlist_675408.html
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