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磷酸化B-Raf抗体，Anti-phospho-B-Raf (Ser365)，一抗磷酸化抗体 价格优惠 上海现货
点击次数：404发布时间：2012/7/23 0:32:28
更新日期：2013/6/8 9:27:24
所 在 地：其它
产品型号：
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详细内容
磷酸化B-Raf抗体Anti-phospho-B-Raf （Ser365）94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; BRAF
360TIYO.COM-上海田源公司
性能参数：
中文名称：磷酸化B-Raf抗体
英文名称：Anti-phospho-B-Raf （Ser365）
别名：94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; BRAF
包装号：G320
产地：中国大陆/进口产品
商标/品牌：Santa、Abcam、MBL、BD、Cell Signaling Technology
供应商：田源试剂 TR、新试剂 NR
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产品详细描述
产品介绍：The Raf kinases are important intermediates in signal transduction. Raf protein family members, including A Raf ａnd B Raf, have intrinsic serine/threonine kinase activity. Interaction between Ras proteins ａnd Raf proteins results in Raf-mediated phosphorylation ａnd activation of MEK （also known as MAP kinase kinase）. Defects in BRAF are involved in a wide range of cancers. B-Raf is a serine/threonine protein kinase that acts as a signal transducer from membrane-associated receptors to nuclear transcription factors. 1 BRAF is important for the regulation of cell proliferation ａnd determination of cell fate during embryogenesis. BRAF acts downstream of Ras ａnd upstream of MEK in the Ras-Raf-MEK-ERK signal transduction pathway, which is a conserved RAS-activated protein kinase cascade that regulates cell growth, proliferation, ａnd differentiation in response to growth factors, cytokines, ａnd hormones.Subcellular Location : Nucleus. Cytoplasm. Cell membrane.Tissue Specificity : Brain ａnd testis.Similarity : Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 protein kinase domain.Contains 1 RBD （Ras-binding） domain.Subunit : Monomer. Homodimer. Heterodimerizes with RAF1, ａnd the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated ａnd enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-753. Found in a complex with at least BRAF, HRAS1, MAP2K1, MAPK3 ａnd RGS14. Interacts with RIT1. Interacts （via N-terminus） with RGS14 （via RBD domains）; the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1 （By similarity）. Interacts with DGKH.BRAF蛋白是有丝分裂原活化的蛋白激酶/细胞外信号调节激酶途径蛋白。正常的B-Raf蛋白的功能是传递来自细胞膜的信号,在Ras-Raf-MEK-ERK信号转导调节途径中有着重要的作用.BRAF是一种在癌细胞的生长和存活中起到关键作用的蛋白质,并且在大多数恶性黑素瘤患者和少数结肠癌、乳腺癌和肺癌患者中发生突变，目前BRAF也用于恶性黑素瘤和肿瘤的研究。
磷酸化B-Raf抗体的质量标准：
| ■其它名称： | 94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; BRAF |
| ■交叉反应： | hu, mo, rat, dog, cow, chk, hrs, pig, dog, Rb |
| ■产品类型： | 一抗磷酸化抗体 |
| ■应用领域: | /肿瘤/细胞生物/免疫学/信号转导/细胞凋亡/转录调节因子/激酶和磷酸酶 |
| ■蛋白分子： | predicted molecular weight: 94kDa |
| ■浓度值： | 1mg/1ml |
| ■物理性状： | Lyophilized or Liquid |
| ■免疫原： | KLH conjugated Synthesised phosphopeptide derived from human B Raf around the phosphorylation site of Ser365 |
| ■亚型： | polyclonal |
| ■纯化方法： | affinity purified by Protein A |
| ■保存液： | 0.01M PBS, pH 7.4 with 10 mg/ml BSA ａnd 0.1% Sodium azide |
| ■应用技术： | //WB=1:100-500//ELISA=1:500-1000//IP=1:20-100//IHC-P=1:100-500//IHC-F=1:100-500//IF=1:100-500（石蜡切片需做抗原修复）/not yet tested in other applications./optimal dilutions/concentrations should be determined by the end user. |
| ■保存环境： | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
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磷酸化B-Raf抗体Anti-phospho-B-Raf （Ser365）的相关产品目录：
1mg/1ml 磷酸化B-Raf抗体 Anti-phospho-B-Raf （Ser365） 94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; BRAF 的来源为Rabbit，其相关产品：免疫组化常用试剂免疫印迹常用试剂各种标记二抗
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