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小鼠抗聚组氨酸单克隆抗体Anti-His tag(2D5)HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His
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中文名称:小鼠抗聚组氨酸单克隆抗体
英文名称:Anti-His tag(2D5)
别名:HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His
包装号:G320
产地:中国大陆/进口产品
商标/品牌:Santa、Abcam、MBL、BD、Cell Signaling Technology
供应商:田源试剂 TR、新试剂 NR
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产品详细描述
产品介绍:2D5The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. 蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白1) 6xHis与不同的Sepharose纯化填料结合(捆绑),可不受构象的影响,在天然或变形的条件下可进行进一步纯化。2) 可在温和的洗脱液环境中,其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。3) 6xHis标签比普通(Tag)要小得多,经纯化后的蛋白可直接进行下游操作。可用于任何表达系统(包括:细菌,杆状病毒和哺乳动物)4) 6xHis标签在生理溶液pH下不带电荷,其标签不会干涉重组蛋白的结构和功能, 6xHis不会影响蛋白分泌。5) 6xHis标签免疫原性差,重组蛋白可以不必除去标签而直接作为抗原进行免疫。小鼠抗聚组氨酸单克隆抗体的质量标准:
■其它名称: | HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His |
■交叉反应: | fusion protein, HHHHHH tagged |
■产品类型: | 一抗标签抗体 |
■应用领域: | /细胞生物/免疫学 |
■蛋白分子: | |
■浓度值: | 1mg/1ml |
■物理性状: | Lyophilized or Liquid |
■免疫原: | KLH conjugated HHHHHH peptide |
■亚型: | monoclonal |
■纯化方法: | affinity purified by Protein A |
■保存液: | 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide |
■应用技术: | //WB=1:100-500//ELISA=1:500-1000//IP=1:20-100//IHC-P=1:100-500//IHC-F=1:100-500//IF=1:100-500(石蜡切片需做抗原修复)/not yet tested in other applications./optimal dilutions/concentrations should be determined by the end user. |
■保存环境: | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
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小鼠抗聚组氨酸单克隆抗体Anti-His tag(2D5)的相关产品目录:
1mg/1ml 小鼠抗聚组氨酸单克隆抗体 Anti-His tag(2D5) HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His的来源为Mouse,其相关产品:免疫组化常用试剂免疫印迹常用试剂各种标记二抗
查询其它抗体目录请点击本公司化工仪器网链接:http://www.chem17.com/st233816/erlist_675408.html
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