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磷酸化细胞核因子抗体 田源一抗磷酸化抗体
点击次数:109发布时间:2012/8/9 11:57:48
更新日期:2013/6/6 11:13:13
所 在 地:其它
产品型号:Anti-phospho-NFKB p65(Thr435)
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详细内容
磷酸化细胞核因子抗体Anti-phospho-NFKB p65(Thr435)NFkB-p65(Phospho-Thr435); RELA(phospho T435); NFKBp65; NF-κBp65; p65 NF kappaB; p65 NFkB;
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性能参数:
中文名称:磷酸化细胞核因子抗体
英文名称:Anti-phospho-NFKB p65(Thr435)
别名:NFkB-p65(Phospho-Thr435); RELA(phospho T435); NFKBp65; NF-κBp65; p65 NF kappaB; p65 NFkB;
包装号:G320
产地:中国大陆/进口产品
商标/品牌:Santa、Abcam、MBL、BD、Cell Signaling Technology
供应商:田源试剂 TR、新试剂 NR
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产品详细描述
产品介绍:p65 is a subunit of the nuclear factor kappa-B, a second messenger, which activates the transcription of a number of genes in multiple tissues. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. [SUBUNIT] Active NF-kappa-B is a heterodimer of an about 50 kDa DNA-binding subunit and the weak DNA-binding subunit p65. Two heterodimers might form a labile tetramer. Subcellular location is at Nucleus, Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Tissue Specificity: Spleen; lower level in brain.NF-κBp65是一种重要的转录因子,NF-kBp65可激活参与炎症、细胞增殖、细胞凋亡等基因的调节,影响着细胞的凋亡,同时影响着肿瘤细胞对细胞毒性药物及离子辐射的敏感性。ras基因诱导的致癌突变作用需NFkB的活化,提示NFkB在致癌发生方面可能起一定作用;另有文献报道,在乳腺癌、非小细胞性肺癌、甲状腺癌、T或B淋巴细胞白血病及病毒诱变导致的肿瘤等人类肿瘤中,NFkB活化或表达。NF-кB可以保护细胞免受肿瘤坏死因子以及电离辐射等引起的凋亡作用,而抑制NFkB的表达可以增加TNF等引起的细胞凋亡,以及增加化疗及放疗对肿瘤细胞的敏感性。磷酸化细胞核因子抗体的质量标准:
■其它名称: | NFkB-p65(Phospho-Thr435); RELA(phospho T435); NFKBp65; NF-κBp65; p65 NF kappaB; p65 NFkB; |
■交叉反应: | hu, mo, rat |
■产品类型: | 一抗磷酸化抗体 |
■应用领域: | /肿瘤/免疫学/染色质和核信号/信号转导/细胞凋亡/转录调节因子/激酶和磷酸酶 |
■蛋白分子: | predicted molecular weight: 61kDa |
■浓度值: | 1mg/1ml |
■物理性状: | Lyophilized or Liquid |
■免疫原: | KLH conjugated Synthesised phosphopeptide derived from human NFKBp65 around the phosphorylation site of Thr435 |
■亚型: | polyclonal |
■纯化方法: | affinity purified by Protein A |
■保存液: | 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide |
■应用技术: | //WB=1:100-500//ELISA=1:500-1000//IP=1:20-100//IHC-P=1:100-500//IHC-F=1:100-500//IF=1:100-500(石蜡切片需做抗原修复)/not yet tested in other applications./optimal dilutions/concentrations should be determined by the end user. |
■保存环境: | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
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磷酸化细胞核因子抗体Anti-phospho-NFKB p65(Thr435)的相关产品目录:
1mg/1ml 磷酸化细胞核因子抗体 Anti-phospho-NFKB p65(Thr435) NFkB-p65(Phospho-Thr435); RELA(phospho T435); NFKBp65; NF-κBp65; p65 NF kappaB; p65 NFkB; 的来源为Rabbit,其相关产品:免疫组化常用试剂免疫印迹常用试剂各种标记二抗
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