产品中文名称：EZ Spin柱式质粒中量抽提试剂盒

产品英文名称：EZ Spin Column Plasmid Medi-Preps Kit

规格：10T

型号：E0118

英文名称：EZ Spin Column Plasmid Medi-Preps Kit其他名称：UNlQ-200柱式质粒中量抽提试剂盒级别：BR规格：10次试剂盒组成：UNIQ-200 Column 10；50-ml Collection Tube 10；RNase A（10mg/ml） 1.6ml；Solution I 110ml；Solution II 110ml；Solution III 2×75ml；Wash solution 24ml；Elution Buffer 15ml产品简介：试剂盒的核心是能特异吸附DNA的UNlQ-200柱。UNlQ-200是一种能放入普通50ml离心管中离心式柱子，柱子的底部有一层惰性大分子材料，它能选择性吸附核酸物质，不吸附蛋白质、多糖和其他非核酸物质。DNA与UNlQ-200的结合需要一定的离子强度。抽提质粒时，细菌采用常规的碱裂解法，离心除去基因组DNA。含质粒的上清液加入到UNIQ-200柱中，经简单的洗涤过步骤除去非特异性结合的杂质。抽提的质粒可用试剂盒提供的Elution Buffer或自备的TE、水洗脱。原理与方法基本和UNlQ-10柱式质粒小量抽提试剂盒相同，只是提取质粒DNA的量比较大，一次可完成200ug质粒的提取。产品特点：1. 中量制备质粒DNA2. 快速，每个抽提可以在40分钟内完成3. 不需要酚抽提和乙醇沉淀、CsC1离心4. 质粒纯度高，洗脱体积小，适合于为细胞转染实验制备DNA用途：生化研究。仅供科研。从工程细菌中中量抽提、纯化质粒DNA，也适用于从各种溶液中浓缩和纯化质粒DNA。保存：RT                                                                              

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