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| Catalog Number: | A0406 |
| Amount: | 50μg/50μl, 100μg/100μl, 200μg/200μl | Price: | ￥4,008/100μg, ￥2,238/50μg | －－>
| Swiss-Prot No.: | P31749 |
| All Names: | AKT, AKT1 kinase, C-AKT, PKB, PKB-alpha, Protein kinase B, RAC, RAC-PK-alpha, RAC-alpha serine/threonine kinase, kinase Akt1 |
| Form of Antibody: | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Storage/Stability: | Store at -20℃/1 year |
| Clonality: | polyclonal |
| Immunogen: | The antiserum was produced against synthesized phosphopeptide derived from human AKT1 around the phosphorylation site of threonine 450 （T-I-TP-P-P）. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Specificity/Sensitivity: | Akt （Phospho-Thr450） antibody detects endogenous levels of AKT1only when phosphorylated at threonine 450. |
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:1000 |
| The optimal working dilution should be determined by the end user. | |
| References: | Payaningal R. Somanath, J. Biol. Chem., Jun 2007; 10.1074.Xiaoming Ju, PNAS, May 2007; 104: 7438 - 7443. Brian DeBosch, Circulation, May 2006; 113: 2097 - 2104. Guilhem Desbrosses, J. Exp. Bot., Feb 2003; 54: 781 - 788. |
| WB: |  Western blot analysis of extracts from NIH/3T3 cells, treated with PDGF （50ng/ml, 20mins）, using Akt （Phospho-Thr450） antibody. The lane on the right is treated with the synthesized peptide. |
| IHC: |  Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Akt （Phospho-Thr450） antibody. The picture on the right is treated with the synthesized peptide. |
