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| Catalog Number: | A0572 |
| Amount: | 50μg/50μl, 100μg/100μl, 200μg/200μl | Price: | ￥4,008/100μg, ￥2,238/50μg | －－>
| Swiss-Prot No.: | Q15637 |
| All Names: | BBP, CW17, Mammalian branch point binding protein mBBP, SF01, Splicing factor 1, Splicing factor SF1, Transcription factor ZFM1, ZFM1, ZFP162, ZNF162, Zinc finger gene in MEN1 locus, Zinc finger protein 162, Mzfm |
| Form of Antibody: | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Storage/Stability: | Store at -20℃/1 year |
| Clonality: | polyclonal |
| Immunogen: | The antiserum was produced against synthesized phosphopeptide derived from human SF1 around the phosphorylation site of serine 82 （S-P-SP-P-E）. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Specificity/Sensitivity: | SF1 （Phospho-Ser82） antibody detects endogenous levels of SF1 only when phosphorylated at serine 82. |
| Reactivity: | Human, Mouse |
| Applications: | WB: 1:500~1:3000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 |
| The optimal working dilution should be determined by the end user. | |
| References: | Susan Y. Park, Development, May 2005; 132: 2415 - 2423. Dujin Zhou, Mol. Endocrinol., Jul 2000; 14: 986. Dagmar Wilhelm, Genes & Dev., Jul 2002; 16: 1839. Philipp Gut,Development, Oct 2005; 132: 4611 - 4619. |
| WB: |  Western blot analysis of extracts from HUVEC cells, treated with anisomycin （25ug/ml, 30mins）, using SF1 （Phospho-Ser82） antibody. The lane on the right is treated with the synthesized peptide. |
| IHC: |  Immunohistochemistry analysis of paraffin-embedded human brain tissue using SF1 （Phospho-Ser82） antibody The picture on the right is treated with the synthesized peptide. |
| IF: | N/A |
| For research use only | |
