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| Catalog Number: | A0629 |
| Amount: | 50μg/50μl, 100μg/100μl, 200μg/200μl | Price: | ￥4,008/100μg, ￥2,238/50μg | －－>
| Swiss-Prot No.: | O14757 |
| All Names: | CHEK1; Chk1; EC 2.7.11.1; kinase Chk1 |
| Form of Antibody: | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Storage/Stability: | Store at -20℃/1 year |
| Clonality: | polyclonal |
| Immunogen: | The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 296 （I-Q-SP-N-L）. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Specificity/Sensitivity: | Chk1 （Phospho-Ser296） antibody detects endogenous levels of Chk1 only when phosphorylated at serine 296. |
| Reactivity: | Human （Identities = 100%, Positives = 100%） |
| Applications: | WB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:5000 |
| The optimal working dilution should be determined by the end user. | |
| References: | Sanchez Y., Science 277:1497-1501（1997）.Flaggs G., Curr. Biol. 7:977-986（1997）.Semba S., Int. J. Oncol. 16:731-737（2000）. |
| WB: |  Western blot analysis of extracts from HUVEC cells, treated with UV （15mins）, using Chk1 （Phospho-Ser296） antibody. The lane on the right is treated with the synthesized peptide. |
| IHC: |  Immunohistochemistry analysis of paraffin-embedded human brain tissue using Chk1 （Phospho-Ser296） antibody. The picture on the right is treated with the synthesized peptide. |
| IF: | N/A |
| For research use only | |
