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| Catalog Number: | A1004 |
| Amount: | 50μg/50μl, 100μg/100μl, 200μg/200μl | Price: | ￥4,008/100μg, ￥2,238/50μg | －－>
| Swiss-Prot No.: | P84022 |
| All Names: | JV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, Smad 3 |
| Form of Antibody: | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Storage/Stability: | Store at -20℃/1 year |
| Clonality: | polyclonal |
| Immunogen: | The antiserum was produced against synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of serine 204 （A-G-SP-P-N）. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Specificity/Sensitivity: | Smad3 （Phospho-Ser204） antibody detects endogenous levels of Smad3 only when phosphorylated at serine 204. |
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB: 1:500~1:3000 ELISA: 1:20000 |
| The optimal working dilution should be determined by the end user. | |
| References: | Melhuish T.A., J. Biol. Chem. 276:32109-32114（2001）.Wu K., J. Biol. Chem. 278:51673-51684（2003）.Wang H., J. Biol. Chem. 280:5154-5162（2005） |
| WB: |  Western blot analysis of extracts from NIH/3T3 cells, treated with Serum （20%, 15mins）, using Smad3 （Phospho-Ser204） antibody. The lane on the right is treated with the synthesized peptide. |
| IHC: | N/A |
| IF: | N/A |
| For research use only | |
