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CREB (Phospho-Ser133) Antibody
点击次数:286发布时间:2012/7/17 15:30:17
更新日期:2012/7/17 15:30:17
所 在 地:美洲
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Catalog Number: | A7053 |
Amount: | 50μg/50μl, 100μg/100μl, 200μg/200μl | Price: | ¥4,008/100μg, ¥2,388/50μg | -->
Swiss-Prot No.: | P16220 |
All Names: | active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB-1; CREB1; Cyclic AMP-responsive element-binding protein 1; MGC9284; transactivator protein |
Form of Antibody: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Storage/Stability: | Store at -20鈩?1 year |
Clonality: | polyclonal |
Immunogen: | The antiserum was produced against synthesized phosphopeptide derived from human CREB around the phosphorylation site of serine 133 (R-P-SP-Y-R). |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity/Sensitivity: | CREB (phospho-Ser133) antibody detects endogenous levels of CREB only when phosphorylated at serine 133. |
Reactivity: | Human, Mouse, Rat |
Applications: | WB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:10000 IP: Various Dilution |
The optimal working dilution should be determined by the end user. | |
References: | Xing J, et al. (1998) Mol Cell Biol 18(4): 1946-55.Tan Y, et al. (1996) EMBO J; 15(17): 4629-42.Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385.Mayo LD, et al. (2001) Biol Chem; 276(27): 25184-9. |
WB: | Western blot analysis of extracts from JK cells and 293 cells, using CREB (phospho-Ser133) antibody. The lane on the right is treated with the synthesized peptide. |
IHC: | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using CREB (phospho-Ser133) antibody. The picture on the right is treated with the synthesized peptide. |
IF: | N/A |
For research use only |