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HDAC2 (Phospho-Ser394) Antibody
点击次数:353发布时间:2012/7/17 15:31:58
更新日期:2012/7/17 15:31:58
所 在 地:美洲
产品型号:
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详细内容
Catalog Number: | A7099 |
Amount: | 50μg/50μl, 100μg/100μl, 200μg/200μl | Price: | ¥4,008/100μg, ¥2,238/50μg | -->
Swiss-Prot No.: | Q92769 |
All Names: | HD2; histone deacetylase 2; YY1 transcription factor binding protein; YY1BP |
Form of Antibody: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Storage/Stability: | Store at -20℃/1 year |
Clonality: | polyclonal |
Immunogen: | The antiserum was produced against synthesized phosphopeptide derived from human HDAC2 around the phosphorylation site of serine 394 (E-D-SP-G-D). |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity/Sensitivity: | HDAC2 (Phospho-Ser394) antibody detects endogenous levels of HDAC2 only when phosphorylated at serine 394. |
Reactivity: | Human, Mouse |
Applications: | WB: 1:500~1:3000 ELISA: 1:20000 |
The optimal working dilution should be determined by the end user. | |
References: | Yang W.-M., Proc. Natl. Acad. Sci. U.S.A. 93:12845-12850(1996).Mungall A.J., Nature 425:805-811(2003).Schmidt D.R., Biochemistry 38:14711-14717(1999). |
WB: | Western blot analysis of extracts from 293 cells, COS cells and HeLa cells, all treated with PMA (125ng/ml, 30mins), using HDAC2 (Phospho-Ser394) antibody. The lane on the right is treated with the synthesized peptide. |
IHC: | N/A |
IF: | N/A |
For research use only |