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厦门慧嘉生物专业销售CYT-352 Leptin tA Human/ 重组人瘦素拮抗剂（三倍突变体） Santa Abcam Cst jackson Pierce Sigma Amresco Qiagen Cayman abnova millipore invitrogen merk ebioscience prospec peprotech细胞因子特价。等生物试剂产品。实验为大，诚信经营，为客户提供“质量的产品”和“质的服务"QQ: 1193953234 1048735792 或登陆（向客服人员索取原版说明书） http://www.biohj.com 欢迎广大老师来询！
Leptin tA Human/ 重组人瘦素拮抗剂（三倍突变体）
产品编号：CYT-352
产品规格：10μg/50μg/1mg
产品规格：10μg/50μg/1mg
| Description | Leptin Antagonist Triple Mutant Human Recombinant is a single non-glycosilated polypeptide chain containing 146 amino ａnd additional Ala at N-terminus acids ａnd having a molecular weight of 16 kDa, Leptin was mutated, resulting in L39A/D40A/F41A. Leptin Antagonist Triple Mutant Human Recombinant was purified by proprietary chromatographic techniques. |
| Source | Escherichia coli. |
| Physical Appearance | White lyophilized （freeze-dried） powder. |
| Formulation | The protein was lyophilized from a concentrated （1mg/ml） solution with 0.0045mM NaHCO3. |
| Solubility | It is recommended to reconstitute the lyophilized Leptin Antagonist Triple Mutant Human Recombinant in sterile 0.4% NaHCO3adjusted to pH 8-9, not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Leptin Antagonist Triple Mutant Human Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 mg/ml ａnd up to 2 mM ａnd filter sterilization Leptin mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein （0.1% HSA or BSA） is suggested. Please prevent freeze-thaw cycles. |
| Purity | Greater than 98.0% as determined by（a） Gel filtration analysis. （b） Analysis by SDS-PAGE. |
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined ａnd was found to be Ala-Val-Pro-Ile-Gln. |
| Biological Activity | ProSpec’s Leptin triple antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays. |
| Protein content | Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.88 as the extinction coefficient for a 0.1% （1mg/ml） solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences （IntelliGenetics）. |
| Usage | ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
