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Trypsin Human, Yeast /重组人胰蛋白酶，酵母
产品编号：PRO-786
产品规格：1mg/5mg/50mg
Introduction
Trypsin （EC3.4.21.4） is part of the serine protease family. Trypsin cleaves lysine ａnd arginine at the C-terminal side of the peptide. The hydrolysis rate is slower if an acidic residue is on either sides of the cleavage site ａnd no cleavage occurs if a proline residue is on the carboxyl side of the cleavage site. Trypsin optimum pH is pH-7 to 9. Trypsin will also hydrolyze ester ａnd amide linkages of synthetic derivatives of amino acids such as: benzoyl L-arginine ethyl ester （BAEE）, p-toluenesulfonyl- L-arginine methyl ester （TAME）, tosyl-L-arginine methyl ester, N-α-benzoyl-L-arginine p-nitroanilide （BAPNA）, L-lysyl-p-nitroanilide, ａnd benzoyl-L-tyrosine ethyl ester （BTEE）. Serine protease inhibitors that inhibit recombinant trypsin include TLCK （N-p-tosyl-L-lysine chloromethyl ketone）, PMSF （phenylmethanesulfonyl fluoride）, benzamidine, soybean trypsin inhibitor, ａnd ovomucoid.
Description
Recombinant Human Trypsin is free from any animal ａnd human sources. Recombinant Human Trypsin expressed in Yeast ａnd purified by standard chromatography techniques. Recombinant Human Trypsin is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Human Trypsin is free from protease inhibitors such as PMSF ａnd EDTA.
Source
Pichia Pastoris.
Physical Appearance
Sterile Filtered clear liquid solution.
Formulation
The protein is formulated with 1mM HCl ａnd 20mM CaCl2, pH-3.
Stability
Recombinant Human Trypsin should be stored at 2-8?C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Biological Activity
2,500 BAEE units/mg powder.
Amino Acid Sequence
IVGGYNCEENSVPYQVSLNSGYHFCGGSLINEQWVVSAGHCYKSRIQ VRLGEHNIEVLEGNEQFINAAKIIRHPQYDRKTLNNDIMLIKLSSRA VINARVSTISLPTAPPATGTKCLISGWGNTASSGADYPDELQCLDAP VLSQAKCEASYPGKITSNMFCVGFLEGGKDSCQGDSGGPVVCNGQLQ GVVSWGDGCAQKNKPGVYTKVYNYVKWIKNTIAANS.
Usage
ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Trypsin Human, Yeast /重组人胰蛋白酶，酵母
产品编号：PRO-786
产品规格：1mg/5mg/50mg
Introduction
Trypsin （EC3.4.21.4） is part of the serine protease family. Trypsin cleaves lysine ａnd arginine at the C-terminal side of the peptide. The hydrolysis rate is slower if an acidic residue is on either sides of the cleavage site ａnd no cleavage occurs if a proline residue is on the carboxyl side of the cleavage site. Trypsin optimum pH is pH-7 to 9. Trypsin will also hydrolyze ester ａnd amide linkages of synthetic derivatives of amino acids such as: benzoyl L-arginine ethyl ester （BAEE）, p-toluenesulfonyl- L-arginine methyl ester （TAME）, tosyl-L-arginine methyl ester, N-α-benzoyl-L-arginine p-nitroanilide （BAPNA）, L-lysyl-p-nitroanilide, ａnd benzoyl-L-tyrosine ethyl ester （BTEE）. Serine protease inhibitors that inhibit recombinant trypsin include TLCK （N-p-tosyl-L-lysine chloromethyl ketone）, PMSF （phenylmethanesulfonyl fluoride）, benzamidine, soybean trypsin inhibitor, ａnd ovomucoid.
Description
Recombinant Human Trypsin is free from any animal ａnd human sources. Recombinant Human Trypsin expressed in Yeast ａnd purified by standard chromatography techniques. Recombinant Human Trypsin is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Human Trypsin is free from protease inhibitors such as PMSF ａnd EDTA.
Source
Pichia Pastoris.
Physical Appearance
Sterile Filtered clear liquid solution.
Formulation
The protein is formulated with 1mM HCl ａnd 20mM CaCl2, pH-3.
Stability
Recombinant Human Trypsin should be stored at 2-8?C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Biological Activity
2,500 BAEE units/mg powder.
Amino Acid Sequence
IVGGYNCEENSVPYQVSLNSGYHFCGGSLINEQWVVSAGHCYKSRIQ VRLGEHNIEVLEGNEQFINAAKIIRHPQYDRKTLNNDIMLIKLSSRA VINARVSTISLPTAPPATGTKCLISGWGNTASSGADYPDELQCLDAP VLSQAKCEASYPGKITSNMFCVGFLEGGKDSCQGDSGGPVVCNGQLQ GVVSWGDGCAQKNKPGVYTKVYNYVKWIKNTIAANS.
Usage
ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Trypsin Human, Yeast /重组人胰蛋白酶，酵母
产品规格：1mg/5mg/50mg
Introduction
Trypsin （EC3.4.21.4） is part of the serine protease family. Trypsin cleaves lysine ａnd arginine at the C-terminal side of the peptide. The hydrolysis rate is slower if an acidic residue is on either sides of the cleavage site ａnd no cleavage occurs if a proline residue is on the carboxyl side of the cleavage site. Trypsin optimum pH is pH-7 to 9. Trypsin will also hydrolyze ester ａnd amide linkages of synthetic derivatives of amino acids such as: benzoyl L-arginine ethyl ester （BAEE）, p-toluenesulfonyl- L-arginine methyl ester （TAME）, tosyl-L-arginine methyl ester, N-α-benzoyl-L-arginine p-nitroanilide （BAPNA）, L-lysyl-p-nitroanilide, ａnd benzoyl-L-tyrosine ethyl ester （BTEE）. Serine protease inhibitors that inhibit recombinant trypsin include TLCK （N-p-tosyl-L-lysine chloromethyl ketone）, PMSF （phenylmethanesulfonyl fluoride）, benzamidine, soybean trypsin inhibitor, ａnd ovomucoid.
Description
Recombinant Human Trypsin is free from any animal ａnd human sources. Recombinant Human Trypsin expressed in Yeast ａnd purified by standard chromatography techniques. Recombinant Human Trypsin is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Human Trypsin is free from protease inhibitors such as PMSF ａnd EDTA.
Source
Pichia Pastoris.
Physical Appearance
Sterile Filtered clear liquid solution.
Formulation
The protein is formulated with 1mM HCl ａnd 20mM CaCl2, pH-3.
Stability
Recombinant Human Trypsin should be stored at 2-8?C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Biological Activity
2,500 BAEE units/mg powder.
Amino Acid Sequence
IVGGYNCEENSVPYQVSLNSGYHFCGGSLINEQWVVSAGHCYKSRIQ VRLGEHNIEVLEGNEQFINAAKIIRHPQYDRKTLNNDIMLIKLSSRA VINARVSTISLPTAPPATGTKCLISGWGNTASSGADYPDELQCLDAP VLSQAKCEASYPGKITSNMFCVGFLEGGKDSCQGDSGGPVVCNGQLQ GVVSWGDGCAQKNKPGVYTKVYNYVKWIKNTIAANS.
Usage
ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
