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PRO-868 RAD1 Human Recombinant
点击次数：186发布时间：2012/7/18 15:30:20
更新日期：2024/10/16 15:58:55
所 在 地：美洲
产品型号：
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详细内容
RAD1 Human Recombinant
产品编号：PRO-868
产品规格：2μg/10μg/1mg
Synonyms
RAD-1, HRAD1, REC1, Cell cycle checkpoint protein RAD1, hRAD1, EC=3.1.11.2, DNA repair exonuclease rad1 homolog, Rad1-like DNA damage checkpoint protein.
Introduction
RAD1 is part of a heterotrimeric cell cycle checkpoint complex, recognized as the 9-1-1 complex, that is activated to stop cell cycle progression in reaction to DNA damage or incomplete DNA replication. RAD1 complex withholds the Rad9 ａnd Hus1 proteins ａnd takes part in cellular responses to DNA damage, by associating with Rad17 ａnd several components of the PCNA-loading heteropentamer, replication factor C. RAD1 takes part in DNA repair ａnd recruited to DNA lesion upon damage by the RAD17-replication factor C （RFC） clamp loader complex. RAD1 takes part as a sliding clamp platform on DNA for several proteins that play a role in long-patch base excision repair （LP-BER）. RAD1 complex stimulates DNA polymerase beta （POLB） activity by raising its affinity for the 3＇＇-OH end of the primer-template ａnd stabilizes POLB to those sites where LP-BER proceeds.
Description
RAD1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 301 amino acids （1-282 a.a.） ａnd having a molecular mass of 33.9 kDa. The RAD1 is fused to 20 amino acid His-Tag at N-terminus ａnd purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
RAD1 Human solution containing 20mM Tris pH-8, 1mM DTT, 0.2M NaCl & 10% glycerol.
Stability
RAD1 Human although stable at 4°C for 1 week, should be stored desiccated
below -18°C.
Please prevent freeze thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL DCLSIFGSSP MPGTLTALRM CYQGYGYPLM LFLEEGGVVT VCKINTQEPE ETLDFDFCST NVINKIILQS
EGLREAFSEL DMTSEVLQIT MSPDKPYFRL STFGNAGSSH LDYPKDSDLM EAFHCNQTQV NRYKISLLKP STKALVLSCK VSIRTDNRGF LSLQYMIRNE DGQICFVEYY CCPDEEVPES ES.
Usage
ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
RAD1 Human Recombinant
产品编号：PRO-868
产品规格：2μg/10μg/1mg
Synonyms
RAD-1, HRAD1, REC1, Cell cycle checkpoint protein RAD1, hRAD1, EC=3.1.11.2, DNA repair exonuclease rad1 homolog, Rad1-like DNA damage checkpoint protein.
Introduction
RAD1 is part of a heterotrimeric cell cycle checkpoint complex, recognized as the 9-1-1 complex, that is activated to stop cell cycle progression in reaction to DNA damage or incomplete DNA replication. RAD1 complex withholds the Rad9 ａnd Hus1 proteins ａnd takes part in cellular responses to DNA damage, by associating with Rad17 ａnd several components of the PCNA-loading heteropentamer, replication factor C. RAD1 takes part in DNA repair ａnd recruited to DNA lesion upon damage by the RAD17-replication factor C （RFC） clamp loader complex. RAD1 takes part as a sliding clamp platform on DNA for several proteins that play a role in long-patch base excision repair （LP-BER）. RAD1 complex stimulates DNA polymerase beta （POLB） activity by raising its affinity for the 3＇＇-OH end of the primer-template ａnd stabilizes POLB to those sites where LP-BER proceeds.
Description
RAD1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 301 amino acids （1-282 a.a.） ａnd having a molecular mass of 33.9 kDa. The RAD1 is fused to 20 amino acid His-Tag at N-terminus ａnd purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
RAD1 Human solution containing 20mM Tris pH-8, 1mM DTT, 0.2M NaCl & 10% glycerol.
Stability
RAD1 Human although stable at 4°C for 1 week, should be stored desiccated
below -18°C.
Please prevent freeze thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL DCLSIFGSSP MPGTLTALRM CYQGYGYPLM LFLEEGGVVT VCKINTQEPE ETLDFDFCST NVINKIILQS
EGLREAFSEL DMTSEVLQIT MSPDKPYFRL STFGNAGSSH LDYPKDSDLM EAFHCNQTQV NRYKISLLKP STKALVLSCK VSIRTDNRGF LSLQYMIRNE DGQICFVEYY CCPDEEVPES ES.
Usage
ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
RAD1 Human Recombinant
产品编号：PRO-868
产品规格：2μg/10μg/1mg
Synonyms
RAD-1, HRAD1, REC1, Cell cycle checkpoint protein RAD1, hRAD1, EC=3.1.11.2, DNA repair exonuclease rad1 homolog, Rad1-like DNA damage checkpoint protein.
Introduction
RAD1 is part of a heterotrimeric cell cycle checkpoint complex, recognized as the 9-1-1 complex, that is activated to stop cell cycle progression in reaction to DNA damage or incomplete DNA replication. RAD1 complex withholds the Rad9 ａnd Hus1 proteins ａnd takes part in cellular responses to DNA damage, by associating with Rad17 ａnd several components of the PCNA-loading heteropentamer, replication factor C. RAD1 takes part in DNA repair ａnd recruited to DNA lesion upon damage by the RAD17-replication factor C （RFC） clamp loader complex. RAD1 takes part as a sliding clamp platform on DNA for several proteins that play a role in long-patch base excision repair （LP-BER）. RAD1 complex stimulates DNA polymerase beta （POLB） activity by raising its affinity for the 3＇＇-OH end of the primer-template ａnd stabilizes POLB to those sites where LP-BER proceeds.
Description
RAD1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 301 amino acids （1-282 a.a.） ａnd having a molecular mass of 33.9 kDa. The RAD1 is fused to 20 amino acid His-Tag at N-terminus ａnd purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
RAD1 Human solution containing 20mM Tris pH-8, 1mM DTT, 0.2M NaCl & 10% glycerol.
Stability
RAD1 Human although stable at 4°C for 1 week, should be stored desiccated
below -18°C.
Please prevent freeze thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL DCLSIFGSSP MPGTLTALRM CYQGYGYPLM LFLEEGGVVT VCKINTQEPE ETLDFDFCST NVINKIILQS
EGLREAFSEL DMTSEVLQIT MSPDKPYFRL STFGNAGSSH LDYPKDSDLM EAFHCNQTQV NRYKISLLKP STKALVLSCK VSIRTDNRGF LSLQYMIRNE DGQICFVEYY CCPDEEVPES ES.
Usage
ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
RAD1 Human Recombinant
产品规格：2μg/10μg/1mg
Synonyms
RAD-1, HRAD1, REC1, Cell cycle checkpoint protein RAD1, hRAD1, EC=3.1.11.2, DNA repair exonuclease rad1 homolog, Rad1-like DNA damage checkpoint protein.
Introduction
RAD1 is part of a heterotrimeric cell cycle checkpoint complex, recognized as the 9-1-1 complex, that is activated to stop cell cycle progression in reaction to DNA damage or incomplete DNA replication. RAD1 complex withholds the Rad9 ａnd Hus1 proteins ａnd takes part in cellular responses to DNA damage, by associating with Rad17 ａnd several components of the PCNA-loading heteropentamer, replication factor C. RAD1 takes part in DNA repair ａnd recruited to DNA lesion upon damage by the RAD17-replication factor C （RFC） clamp loader complex. RAD1 takes part as a sliding clamp platform on DNA for several proteins that play a role in long-patch base excision repair （LP-BER）. RAD1 complex stimulates DNA polymerase beta （POLB） activity by raising its affinity for the 3＇＇-OH end of the primer-template ａnd stabilizes POLB to those sites where LP-BER proceeds.
Description
RAD1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 301 amino acids （1-282 a.a.） ａnd having a molecular mass of 33.9 kDa. The RAD1 is fused to 20 amino acid His-Tag at N-terminus ａnd purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
RAD1 Human solution containing 20mM Tris pH-8, 1mM DTT, 0.2M NaCl & 10% glycerol.
Stability
RAD1 Human although stable at 4°C for 1 week, should be stored desiccated
below -18°C.
Please prevent freeze thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL DCLSIFGSSP MPGTLTALRM CYQGYGYPLM LFLEEGGVVT VCKINTQEPE ETLDFDFCST NVINKIILQS
EGLREAFSEL DMTSEVLQIT MSPDKPYFRL STFGNAGSSH LDYPKDSDLM EAFHCNQTQV NRYKISLLKP STKALVLSCK VSIRTDNRGF LSLQYMIRNE DGQICFVEYY CCPDEEVPES ES.
Usage
ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
