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CYT-700 Fibroblast Growth Factor-19 Human Recombinant/重组人成纤维细胞生长因子-19
点击次数：226发布时间：2012/7/19 14:38:57
更新日期：2024/10/16 15:58:55
所 在 地：美洲
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详细内容
Fibroblast Growth Factor-19 Human Recombinant/重组人成纤维细胞生长因子-19
产品编号：CYT-700
产品规格：5μg/25μg/1mg
Synonyms
Fibroblast growth factor 19, FGF-19, FGF19.
Introduction
The FGFs are a family of more than 20 small （~17–26 kDa） secreted peptides. The initial characterization of these proteins focused on their ability to stimulate fibroblast proliferation. This mitogenic activity was mediated through FGF receptors （FGFRs） 1, 2, or 3. A fourth closely related tyrosine kinase receptor （FGFR4） was able to bind the FGFs but did not lead to a mitogenic response.
FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors （FGFRs）: FGFR-1, -2, -3, ａnd -4, all with intrinsic tyrosine kinase activity and, except for FGFR-4, multiple splice isoforms, ａnd FGFR-5, which lacks an intracellular kinase domain. There is growing evidence that FGFRs can be important for regulation of glucose ａnd lipid homeostasis. The overexpression of a dominant negative form of FGFR-1 in ? cells leads to diabetes in mice, which thus implies that proper FGF signaling is required for normal ? cell function ａnd glycemia maintenance. FGFR-2 appears to be a key molecule during pancreatic development. Moreover, FGFR-4 has been implicated in cholesterol metabolism ａnd bile acid synthesis.
FGF-19, has been shown to cause resistance to diet-induced obesity ａnd insulin desensitization ａnd to improve insulin, glucose, ａnd lipid profiles in diabetic rodents. Since these effects, at least in part, are mediated through the observed changes in metabolic rates, FGF-19 can be considered as a regulator of energy expenditure.
FGF-21 is preferentially expressed in liver, but an exact knowledge of FGF-21 bioactivity ａnd its mode of action have been lacking to date. FGF-21 is a potent activator of glucose uptake on adipocytes, protects animals from diet-induced obesity when overexpressed in transgenic mice, ａnd lowers blood glucose ａnd triglyceride levels when therapeutically administered to diabetic rodents.
Description
FGF19 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 195 amino acids ａnd having a molecular mass of 21.8 kDa.
The FGF-19 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Filtered white lyophilized powder.
Formulation
Filtered （0.4 μm） ａnd lyophilized from 1mg/ml in 1x PBS, pH 7.4.
Solubility
Add deionized water to a working concentration approximately 0.5 mg/ml ａnd let the lyophilized pellet dissolve completely.
Stability
Lyophilized FGF-19 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibroblast Growth Factor-19 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Biological Activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of balb/c 3T3 cells is 100-150ng/ml.
Purity
Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（c） Analysis by SDS-PAGE.
Amino acid sequence
MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK.
Usage
ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
Fibroblast Growth Factor-19 Human Recombinant/重组人成纤维细胞生长因子-19
产品编号：CYT-700
产品规格：5μg/25μg/1mg
Synonyms
Fibroblast growth factor 19, FGF-19, FGF19.
Introduction
The FGFs are a family of more than 20 small （~17–26 kDa） secreted peptides. The initial characterization of these proteins focused on their ability to stimulate fibroblast proliferation. This mitogenic activity was mediated through FGF receptors （FGFRs） 1, 2, or 3. A fourth closely related tyrosine kinase receptor （FGFR4） was able to bind the FGFs but did not lead to a mitogenic response.
FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors （FGFRs）: FGFR-1, -2, -3, ａnd -4, all with intrinsic tyrosine kinase activity and, except for FGFR-4, multiple splice isoforms, ａnd FGFR-5, which lacks an intracellular kinase domain. There is growing evidence that FGFRs can be important for regulation of glucose ａnd lipid homeostasis. The overexpression of a dominant negative form of FGFR-1 in ? cells leads to diabetes in mice, which thus implies that proper FGF signaling is required for normal ? cell function ａnd glycemia maintenance. FGFR-2 appears to be a key molecule during pancreatic development. Moreover, FGFR-4 has been implicated in cholesterol metabolism ａnd bile acid synthesis.
FGF-19, has been shown to cause resistance to diet-induced obesity ａnd insulin desensitization ａnd to improve insulin, glucose, ａnd lipid profiles in diabetic rodents. Since these effects, at least in part, are mediated through the observed changes in metabolic rates, FGF-19 can be considered as a regulator of energy expenditure.
FGF-21 is preferentially expressed in liver, but an exact knowledge of FGF-21 bioactivity ａnd its mode of action have been lacking to date. FGF-21 is a potent activator of glucose uptake on adipocytes, protects animals from diet-induced obesity when overexpressed in transgenic mice, ａnd lowers blood glucose ａnd triglyceride levels when therapeutically administered to diabetic rodents.
Description
FGF19 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 195 amino acids ａnd having a molecular mass of 21.8 kDa.
The FGF-19 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Filtered white lyophilized powder.
Formulation
Filtered （0.4 μm） ａnd lyophilized from 1mg/ml in 1x PBS, pH 7.4.
Solubility
Add deionized water to a working concentration approximately 0.5 mg/ml ａnd let the lyophilized pellet dissolve completely.
Stability
Lyophilized FGF-19 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibroblast Growth Factor-19 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Biological Activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of balb/c 3T3 cells is 100-150ng/ml.
Purity
Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（c） Analysis by SDS-PAGE.
Amino acid sequence
MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK.
Usage
ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
Fibroblast Growth Factor-19 Human Recombinant/重组人成纤维细胞生长因子-19
产品编号：CYT-700
产品规格：5μg/25μg/1mg
Synonyms
Fibroblast growth factor 19, FGF-19, FGF19.
Introduction
The FGFs are a family of more than 20 small （~17–26 kDa） secreted peptides. The initial characterization of these proteins focused on their ability to stimulate fibroblast proliferation. This mitogenic activity was mediated through FGF receptors （FGFRs） 1, 2, or 3. A fourth closely related tyrosine kinase receptor （FGFR4） was able to bind the FGFs but did not lead to a mitogenic response.
FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors （FGFRs）: FGFR-1, -2, -3, ａnd -4, all with intrinsic tyrosine kinase activity and, except for FGFR-4, multiple splice isoforms, ａnd FGFR-5, which lacks an intracellular kinase domain. There is growing evidence that FGFRs can be important for regulation of glucose ａnd lipid homeostasis. The overexpression of a dominant negative form of FGFR-1 in ? cells leads to diabetes in mice, which thus implies that proper FGF signaling is required for normal ? cell function ａnd glycemia maintenance. FGFR-2 appears to be a key molecule during pancreatic development. Moreover, FGFR-4 has been implicated in cholesterol metabolism ａnd bile acid synthesis.
FGF-19, has been shown to cause resistance to diet-induced obesity ａnd insulin desensitization ａnd to improve insulin, glucose, ａnd lipid profiles in diabetic rodents. Since these effects, at least in part, are mediated through the observed changes in metabolic rates, FGF-19 can be considered as a regulator of energy expenditure.
FGF-21 is preferentially expressed in liver, but an exact knowledge of FGF-21 bioactivity ａnd its mode of action have been lacking to date. FGF-21 is a potent activator of glucose uptake on adipocytes, protects animals from diet-induced obesity when overexpressed in transgenic mice, ａnd lowers blood glucose ａnd triglyceride levels when therapeutically administered to diabetic rodents.
Description
FGF19 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 195 amino acids ａnd having a molecular mass of 21.8 kDa.
The FGF-19 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Filtered white lyophilized powder.
Formulation
Filtered （0.4 μm） ａnd lyophilized from 1mg/ml in 1x PBS, pH 7.4.
Solubility
Add deionized water to a working concentration approximately 0.5 mg/ml ａnd let the lyophilized pellet dissolve completely.
Stability
Lyophilized FGF-19 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibroblast Growth Factor-19 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Biological Activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of balb/c 3T3 cells is 100-150ng/ml.
Purity
Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（c） Analysis by SDS-PAGE.
Amino acid sequence
MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK.
Usage
ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
Fibroblast Growth Factor-19 Human Recombinant/重组人成纤维细胞生长因子-19
产品编号：CYT-700
产品规格：5μg/25μg/1mg
Synonyms
Fibroblast growth factor 19, FGF-19, FGF19.
Introduction
The FGFs are a family of more than 20 small （~17–26 kDa） secreted peptides. The initial characterization of these proteins focused on their ability to stimulate fibroblast proliferation. This mitogenic activity was mediated through FGF receptors （FGFRs） 1, 2, or 3. A fourth closely related tyrosine kinase receptor （FGFR4） was able to bind the FGFs but did not lead to a mitogenic response.
FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors （FGFRs）: FGFR-1, -2, -3, ａnd -4, all with intrinsic tyrosine kinase activity and, except for FGFR-4, multiple splice isoforms, ａnd FGFR-5, which lacks an intracellular kinase domain. There is growing evidence that FGFRs can be important for regulation of glucose ａnd lipid homeostasis. The overexpression of a dominant negative form of FGFR-1 in ? cells leads to diabetes in mice, which thus implies that proper FGF signaling is required for normal ? cell function ａnd glycemia maintenance. FGFR-2 appears to be a key molecule during pancreatic development. Moreover, FGFR-4 has been implicated in cholesterol metabolism ａnd bile acid synthesis.
FGF-19, has been shown to cause resistance to diet-induced obesity ａnd insulin desensitization ａnd to improve insulin, glucose, ａnd lipid profiles in diabetic rodents. Since these effects, at least in part, are mediated through the observed changes in metabolic rates, FGF-19 can be considered as a regulator of energy expenditure.
FGF-21 is preferentially expressed in liver, but an exact knowledge of FGF-21 bioactivity ａnd its mode of action have been lacking to date. FGF-21 is a potent activator of glucose uptake on adipocytes, protects animals from diet-induced obesity when overexpressed in transgenic mice, ａnd lowers blood glucose ａnd triglyceride levels when therapeutically administered to diabetic rodents.
Description
FGF19 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 195 amino acids ａnd having a molecular mass of 21.8 kDa.
The FGF-19 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Filtered white lyophilized powder.
Formulation
Filtered （0.4 μm） ａnd lyophilized from 1mg/ml in 1x PBS, pH 7.4.
Solubility
Add deionized water to a working concentration approximately 0.5 mg/ml ａnd let the lyophilized pellet dissolve completely.
Stability
Lyophilized FGF-19 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibroblast Growth Factor-19 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Biological Activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of balb/c 3T3 cells is 100-150ng/ml.
Purity
Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（c） Analysis by SDS-PAGE.
Amino acid sequence
MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK.
Usage
ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
Fibroblast Growth Factor-19 Human Recombinant/重组人成纤维细胞生长因子-19
产品编号：CYT-700
产品规格：5μg/25μg/1mg
Synonyms
Fibroblast growth factor 19, FGF-19, FGF19.
Introduction
The FGFs are a family of more than 20 small （~17–26 kDa） secreted peptides. The initial characterization of these proteins focused on their ability to stimulate fibroblast proliferation. This mitogenic activity was mediated through FGF receptors （FGFRs） 1, 2, or 3. A fourth closely related tyrosine kinase receptor （FGFR4） was able to bind the FGFs but did not lead to a mitogenic response.
FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors （FGFRs）: FGFR-1, -2, -3, ａnd -4, all with intrinsic tyrosine kinase activity and, except for FGFR-4, multiple splice isoforms, ａnd FGFR-5, which lacks an intracellular kinase domain. There is growing evidence that FGFRs can be important for regulation of glucose ａnd lipid homeostasis. The overexpression of a dominant negative form of FGFR-1 in ? cells leads to diabetes in mice, which thus implies that proper FGF signaling is required for normal ? cell function ａnd glycemia maintenance. FGFR-2 appears to be a key molecule during pancreatic development. Moreover, FGFR-4 has been implicated in cholesterol metabolism ａnd bile acid synthesis.
FGF-19, has been shown to cause resistance to diet-induced obesity ａnd insulin desensitization ａnd to improve insulin, glucose, ａnd lipid profiles in diabetic rodents. Since these effects, at least in part, are mediated through the observed changes in metabolic rates, FGF-19 can be considered as a regulator of energy expenditure.
FGF-21 is preferentially expressed in liver, but an exact knowledge of FGF-21 bioactivity ａnd its mode of action have been lacking to date. FGF-21 is a potent activator of glucose uptake on adipocytes, protects animals from diet-induced obesity when overexpressed in transgenic mice, ａnd lowers blood glucose ａnd triglyceride levels when therapeutically administered to diabetic rodents.
Description
FGF19 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 195 amino acids ａnd having a molecular mass of 21.8 kDa.
The FGF-19 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Filtered white lyophilized powder.
Formulation
Filtered （0.4 μm） ａnd lyophilized from 1mg/ml in 1x PBS, pH 7.4.
Solubility
Add deionized water to a working concentration approximately 0.5 mg/ml ａnd let the lyophilized pellet dissolve completely.
Stability
Lyophilized FGF-19 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibroblast Growth Factor-19 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Biological Activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of balb/c 3T3 cells is 100-150ng/ml.
Purity
Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（c） Analysis by SDS-PAGE.
Amino acid sequence
MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK.
Usage
ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
Fibroblast Growth Factor-19 Human Recombinant/重组人成纤维细胞生长因子-19
产品编号：CYT-700
产品规格：5μg/25μg/1mg
Synonyms
Fibroblast growth factor 19, FGF-19, FGF19.
Introduction
The FGFs are a family of more than 20 small （~17–26 kDa） secreted peptides. The initial characterization of these proteins focused on their ability to stimulate fibroblast proliferation. This mitogenic activity was mediated through FGF receptors （FGFRs） 1, 2, or 3. A fourth closely related tyrosine kinase receptor （FGFR4） was able to bind the FGFs but did not lead to a mitogenic response.
FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors （FGFRs）: FGFR-1, -2, -3, ａnd -4, all with intrinsic tyrosine kinase activity and, except for FGFR-4, multiple splice isoforms, ａnd FGFR-5, which lacks an intracellular kinase domain. There is growing evidence that FGFRs can be important for regulation of glucose ａnd lipid homeostasis. The overexpression of a dominant negative form of FGFR-1 in ? cells leads to diabetes in mice, which thus implies that proper FGF signaling is required for normal ? cell function ａnd glycemia maintenance. FGFR-2 appears to be a key molecule during pancreatic development. Moreover, FGFR-4 has been implicated in cholesterol metabolism ａnd bile acid synthesis.
FGF-19, has been shown to cause resistance to diet-induced obesity ａnd insulin desensitization ａnd to improve insulin, glucose, ａnd lipid profiles in diabetic rodents. Since these effects, at least in part, are mediated through the observed changes in metabolic rates, FGF-19 can be considered as a regulator of energy expenditure.
FGF-21 is preferentially expressed in liver, but an exact knowledge of FGF-21 bioactivity ａnd its mode of action have been lacking to date. FGF-21 is a potent activator of glucose uptake on adipocytes, protects animals from diet-induced obesity when overexpressed in transgenic mice, ａnd lowers blood glucose ａnd triglyceride levels when therapeutically administered to diabetic rodents.
Description
FGF19 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 195 amino acids ａnd having a molecular mass of 21.8 kDa.
The FGF-19 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Filtered white lyophilized powder.
Formulation
Filtered （0.4 μm） ａnd lyophilized from 1mg/ml in 1x PBS, pH 7.4.
Solubility
Add deionized water to a working concentration approximately 0.5 mg/ml ａnd let the lyophilized pellet dissolve completely.
Stability
Lyophilized FGF-19 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibroblast Growth Factor-19 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Biological Activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of balb/c 3T3 cells is 100-150ng/ml.
Purity
Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（c） Analysis by SDS-PAGE.
Amino acid sequence
MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK.
Usage
ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
产品规格：5μg/25μg/1mg
Synonyms
Fibroblast growth factor 19, FGF-19, FGF19.
Introduction
The FGFs are a family of more than 20 small （~17–26 kDa） secreted peptides. The initial characterization of these proteins focused on their ability to stimulate fibroblast proliferation. This mitogenic activity was mediated through FGF receptors （FGFRs） 1, 2, or 3. A fourth closely related tyrosine kinase receptor （FGFR4） was able to bind the FGFs but did not lead to a mitogenic response.
FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors （FGFRs）: FGFR-1, -2, -3, ａnd -4, all with intrinsic tyrosine kinase activity and, except for FGFR-4, multiple splice isoforms, ａnd FGFR-5, which lacks an intracellular kinase domain. There is growing evidence that FGFRs can be important for regulation of glucose ａnd lipid homeostasis. The overexpression of a dominant negative form of FGFR-1 in ? cells leads to diabetes in mice, which thus implies that proper FGF signaling is required for normal ? cell function ａnd glycemia maintenance. FGFR-2 appears to be a key molecule during pancreatic development. Moreover, FGFR-4 has been implicated in cholesterol metabolism ａnd bile acid synthesis.
FGF-19, has been shown to cause resistance to diet-induced obesity ａnd insulin desensitization ａnd to improve insulin, glucose, ａnd lipid profiles in diabetic rodents. Since these effects, at least in part, are mediated through the observed changes in metabolic rates, FGF-19 can be considered as a regulator of energy expenditure.
FGF-21 is preferentially expressed in liver, but an exact knowledge of FGF-21 bioactivity ａnd its mode of action have been lacking to date. FGF-21 is a potent activator of glucose uptake on adipocytes, protects animals from diet-induced obesity when overexpressed in transgenic mice, ａnd lowers blood glucose ａnd triglyceride levels when therapeutically administered to diabetic rodents.
Description
FGF19 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 195 amino acids ａnd having a molecular mass of 21.8 kDa.
The FGF-19 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Filtered white lyophilized powder.
Formulation
Filtered （0.4 μm） ａnd lyophilized from 1mg/ml in 1x PBS, pH 7.4.
Solubility
Add deionized water to a working concentration approximately 0.5 mg/ml ａnd let the lyophilized pellet dissolve completely.
Stability
Lyophilized FGF-19 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibroblast Growth Factor-19 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Biological Activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of balb/c 3T3 cells is 100-150ng/ml.
Purity
Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（c） Analysis by SDS-PAGE.
Amino acid sequence
MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK.
Usage
ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
