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PRO-330 CAPN2/牛钙蛋白酶2催化亚基
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更新日期：2024/10/16 15:58:55
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厦门慧嘉生物专业销售PRO-330 CAPN2/牛钙蛋白酶2催化亚基 Santa Abcam Cst jackson Pierce Sigma Amresco Qiagen Cayman abnova millipore invitrogen merk ebioscience prospec peprotech细胞因子特价。等生物试剂产品。实验为大，诚信经营，为客户提供“质量的产品”和“质的服务"QQ: 1193953234 1048735792 或登陆（向客服人员索取原版说明书） http://www.biohj.com 欢迎广大老师来询！
CAPN2/牛钙蛋白酶2催化亚基
产品编号：PRO-330
产品规格：2μg/5μg/10μg
Synonyms Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2. Introduction Calpain’s activity is attributed to two main isoforms: ?-calpain ａnd m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes ａnd cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 （?-calpain） or Capn2 （m-calpain）, ａnd a common regulatory subunit encoded by Capn4. Calpain2 （M-calpain） is believed to be membrane bound ａnd functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2. Description CAPN2 consists of an 80 kDa large subunit ａnd a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, ａnd Phenyl-Sepharose CL-4B columns. Source Bovine Myocardium. Physical Appearance Sterile Filtered colorless solution. Formulation 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT ａnd 50% glycerol, pH 7.4. Stability CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Applications This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry （final dilution 1:1＇000）.
B- Add 1μg of protein to 1ml of the diluted antibody solution ａnd mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections ａnd incubate for 3 days.
E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）.
As a result, the immunostaining should be strongly reduced or even completely prevented. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
CAPN2/牛钙蛋白酶2催化亚基
产品编号：PRO-330
产品规格：2μg/5μg/10μg
Synonyms Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2. Introduction Calpain’s activity is attributed to two main isoforms: ?-calpain ａnd m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes ａnd cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 （?-calpain） or Capn2 （m-calpain）, ａnd a common regulatory subunit encoded by Capn4. Calpain2 （M-calpain） is believed to be membrane bound ａnd functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2. Description CAPN2 consists of an 80 kDa large subunit ａnd a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, ａnd Phenyl-Sepharose CL-4B columns. Source Bovine Myocardium. Physical Appearance Sterile Filtered colorless solution. Formulation 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT ａnd 50% glycerol, pH 7.4. Stability CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Applications This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry （final dilution 1:1＇000）.
B- Add 1μg of protein to 1ml of the diluted antibody solution ａnd mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections ａnd incubate for 3 days.
E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）.
As a result, the immunostaining should be strongly reduced or even completely prevented. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
CAPN2/牛钙蛋白酶2催化亚基
产品编号：PRO-330
产品规格：2μg/5μg/10μg
Synonyms Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2. Introduction Calpain’s activity is attributed to two main isoforms: ?-calpain ａnd m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes ａnd cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 （?-calpain） or Capn2 （m-calpain）, ａnd a common regulatory subunit encoded by Capn4. Calpain2 （M-calpain） is believed to be membrane bound ａnd functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2. Description CAPN2 consists of an 80 kDa large subunit ａnd a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, ａnd Phenyl-Sepharose CL-4B columns. Source Bovine Myocardium. Physical Appearance Sterile Filtered colorless solution. Formulation 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT ａnd 50% glycerol, pH 7.4. Stability CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Applications This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry （final dilution 1:1＇000）.
B- Add 1μg of protein to 1ml of the diluted antibody solution ａnd mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections ａnd incubate for 3 days.
E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）.
As a result, the immunostaining should be strongly reduced or even completely prevented. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
CAPN2/牛钙蛋白酶2催化亚基
产品编号：PRO-330
产品规格：2μg/5μg/10μg
Synonyms Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2. Introduction Calpain’s activity is attributed to two main isoforms: ?-calpain ａnd m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes ａnd cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 （?-calpain） or Capn2 （m-calpain）, ａnd a common regulatory subunit encoded by Capn4. Calpain2 （M-calpain） is believed to be membrane bound ａnd functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2. Description CAPN2 consists of an 80 kDa large subunit ａnd a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, ａnd Phenyl-Sepharose CL-4B columns. Source Bovine Myocardium. Physical Appearance Sterile Filtered colorless solution. Formulation 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT ａnd 50% glycerol, pH 7.4. Stability CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Applications This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry （final dilution 1:1＇000）.
B- Add 1μg of protein to 1ml of the diluted antibody solution ａnd mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections ａnd incubate for 3 days.
E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）.
As a result, the immunostaining should be strongly reduced or even completely prevented. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
CAPN2/牛钙蛋白酶2催化亚基
产品编号：PRO-330
产品规格：2μg/5μg/10μg
Synonyms Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2. Introduction Calpain’s activity is attributed to two main isoforms: ?-calpain ａnd m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes ａnd cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 （?-calpain） or Capn2 （m-calpain）, ａnd a common regulatory subunit encoded by Capn4. Calpain2 （M-calpain） is believed to be membrane bound ａnd functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2. Description CAPN2 consists of an 80 kDa large subunit ａnd a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, ａnd Phenyl-Sepharose CL-4B columns. Source Bovine Myocardium. Physical Appearance Sterile Filtered colorless solution. Formulation 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT ａnd 50% glycerol, pH 7.4. Stability CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Applications This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry （final dilution 1:1＇000）.
B- Add 1μg of protein to 1ml of the diluted antibody solution ａnd mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections ａnd incubate for 3 days.
E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）.
As a result, the immunostaining should be strongly reduced or even completely prevented. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
CAPN2/牛钙蛋白酶2催化亚基
产品编号：PRO-330
产品规格：2μg/5μg/10μg
Synonyms Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2. Introduction Calpain’s activity is attributed to two main isoforms: ?-calpain ａnd m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes ａnd cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 （?-calpain） or Capn2 （m-calpain）, ａnd a common regulatory subunit encoded by Capn4. Calpain2 （M-calpain） is believed to be membrane bound ａnd functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2. Description CAPN2 consists of an 80 kDa large subunit ａnd a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, ａnd Phenyl-Sepharose CL-4B columns. Source Bovine Myocardium. Physical Appearance Sterile Filtered colorless solution. Formulation 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT ａnd 50% glycerol, pH 7.4. Stability CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Applications This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry （final dilution 1:1＇000）.
B- Add 1μg of protein to 1ml of the diluted antibody solution ａnd mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections ａnd incubate for 3 days.
E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）.
As a result, the immunostaining should be strongly reduced or even completely prevented. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
CAPN2/牛钙蛋白酶2催化亚基
产品编号：PRO-330
产品规格：2μg/5μg/10μg
Synonyms Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2. Introduction Calpain’s activity is attributed to two main isoforms: ?-calpain ａnd m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes ａnd cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 （?-calpain） or Capn2 （m-calpain）, ａnd a common regulatory subunit encoded by Capn4. Calpain2 （M-calpain） is believed to be membrane bound ａnd functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2. Description CAPN2 consists of an 80 kDa large subunit ａnd a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, ａnd Phenyl-Sepharose CL-4B columns. Source Bovine Myocardium. Physical Appearance Sterile Filtered colorless solution. Formulation 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT ａnd 50% glycerol, pH 7.4. Stability CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Applications This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry （final dilution 1:1＇000）.
B- Add 1μg of protein to 1ml of the diluted antibody solution ａnd mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections ａnd incubate for 3 days.
E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）.
As a result, the immunostaining should be strongly reduced or even completely prevented. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
CAPN2/牛钙蛋白酶2催化亚基
产品编号：PRO-330
产品规格：2μg/5μg/10μg
Synonyms Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2. Introduction Calpain’s activity is attributed to two main isoforms: ?-calpain ａnd m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes ａnd cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 （?-calpain） or Capn2 （m-calpain）, ａnd a common regulatory subunit encoded by Capn4. Calpain2 （M-calpain） is believed to be membrane bound ａnd functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2. Description CAPN2 consists of an 80 kDa large subunit ａnd a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, ａnd Phenyl-Sepharose CL-4B columns. Source Bovine Myocardium. Physical Appearance Sterile Filtered colorless solution. Formulation 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT ａnd 50% glycerol, pH 7.4. Stability CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Applications This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry （final dilution 1:1＇000）.
B- Add 1μg of protein to 1ml of the diluted antibody solution ａnd mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections ａnd incubate for 3 days.
E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）.
As a result, the immunostaining should be strongly reduced or even completely prevented. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
CAPN2/牛钙蛋白酶2催化亚基
产品编号：PRO-330
产品规格：2μg/5μg/10μg
Synonyms Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2. Introduction Calpain’s activity is attributed to two main isoforms: ?-calpain ａnd m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes ａnd cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 （?-calpain） or Capn2 （m-calpain）, ａnd a common regulatory subunit encoded by Capn4. Calpain2 （M-calpain） is believed to be membrane bound ａnd functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2. Description CAPN2 consists of an 80 kDa large subunit ａnd a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, ａnd Phenyl-Sepharose CL-4B columns. Source Bovine Myocardium. Physical Appearance Sterile Filtered colorless solution. Formulation 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT ａnd 50% glycerol, pH 7.4. Stability CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Applications This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry （final dilution 1:1＇000）.
B- Add 1μg of protein to 1ml of the diluted antibody solution ａnd mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections ａnd incubate for 3 days.
E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）.
As a result, the immunostaining should be strongly reduced or even completely prevented. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
CAPN2/牛钙蛋白酶2催化亚基
产品编号：PRO-330
产品规格：2μg/5μg/10μg
Synonyms Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2. Introduction Calpain’s activity is attributed to two main isoforms: ?-calpain ａnd m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes ａnd cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 （?-calpain） or Capn2 （m-calpain）, ａnd a common regulatory subunit encoded by Capn4. Calpain2 （M-calpain） is believed to be membrane bound ａnd functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2. Description CAPN2 consists of an 80 kDa large subunit ａnd a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, ａnd Phenyl-Sepharose CL-4B columns. Source Bovine Myocardium. Physical Appearance Sterile Filtered colorless solution. Formulation 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT ａnd 50% glycerol, pH 7.4. Stability CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Applications This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry （final dilution 1:1＇000）.
B- Add 1μg of protein to 1ml of the diluted antibody solution ａnd mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections ａnd incubate for 3 days.
E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）.
As a result, the immunostaining should be strongly reduced or even completely prevented. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
CAPN2/牛钙蛋白酶2催化亚基
产品编号：PRO-330
产品规格：2μg/5μg/10μg
Synonyms Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2. Introduction Calpain’s activity is attributed to two main isoforms: ?-calpain ａnd m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes ａnd cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 （?-calpain） or Capn2 （m-calpain）, ａnd a common regulatory subunit encoded by Capn4. Calpain2 （M-calpain） is believed to be membrane bound ａnd functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2. Description CAPN2 consists of an 80 kDa large subunit ａnd a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, ａnd Phenyl-Sepharose CL-4B columns. Source Bovine Myocardium. Physical Appearance Sterile Filtered colorless solution. Formulation 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT ａnd 50% glycerol, pH 7.4. Stability CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Applications This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry （final dilution 1:1＇000）.
B- Add 1μg of protein to 1ml of the diluted antibody solution ａnd mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections ａnd incubate for 3 days.
E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）.
As a result, the immunostaining should be strongly reduced or even completely prevented. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
CAPN2/牛钙蛋白酶2催化亚基
产品编号：PRO-330
产品规格：2μg/5μg/10μg
Synonyms Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2. Introduction Calpain’s activity is attributed to two main isoforms: ?-calpain ａnd m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes ａnd cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 （?-calpain） or Capn2 （m-calpain）, ａnd a common regulatory subunit encoded by Capn4. Calpain2 （M-calpain） is believed to be membrane bound ａnd functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2. Description CAPN2 consists of an 80 kDa large subunit ａnd a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, ａnd Phenyl-Sepharose CL-4B columns. Source Bovine Myocardium. Physical Appearance Sterile Filtered colorless solution. Formulation 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT ａnd 50% glycerol, pH 7.4. Stability CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Applications This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry （final dilution 1:1＇000）.
B- Add 1μg of protein to 1ml of the diluted antibody solution ａnd mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections ａnd incubate for 3 days.
E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）.
As a result, the immunostaining should be strongly reduced or even completely prevented. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
CAPN2/牛钙蛋白酶2催化亚基
产品编号：PRO-330
产品规格：2μg/5μg/10μg
Synonyms Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2. Introduction Calpain’s activity is attributed to two main isoforms: ?-calpain ａnd m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes ａnd cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 （?-calpain） or Capn2 （m-calpain）, ａnd a common regulatory subunit encoded by Capn4. Calpain2 （M-calpain） is believed to be membrane bound ａnd functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2. Description CAPN2 consists of an 80 kDa large subunit ａnd a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, ａnd Phenyl-Sepharose CL-4B columns. Source Bovine Myocardium. Physical Appearance Sterile Filtered colorless solution. Formulation 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT ａnd 50% glycerol, pH 7.4. Stability CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Applications This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry （final dilution 1:1＇000）.
B- Add 1μg of protein to 1ml of the diluted antibody solution ａnd mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections ａnd incubate for 3 days.
E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）.
As a result, the immunostaining should be strongly reduced or even completely prevented. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
CAPN2/牛钙蛋白酶2催化亚基
产品编号：PRO-330
产品规格：2μg/5μg/10μg
Synonyms Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2. Introduction Calpain’s activity is attributed to two main isoforms: ?-calpain ａnd m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes ａnd cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 （?-calpain） or Capn2 （m-calpain）, ａnd a common regulatory subunit encoded by Capn4. Calpain2 （M-calpain） is believed to be membrane bound ａnd functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2. Description CAPN2 consists of an 80 kDa large subunit ａnd a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, ａnd Phenyl-Sepharose CL-4B columns. Source Bovine Myocardium. Physical Appearance Sterile Filtered colorless solution. Formulation 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT ａnd 50% glycerol, pH 7.4. Stability CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Applications This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry （final dilution 1:1＇000）.
B- Add 1μg of protein to 1ml of the diluted antibody solution ａnd mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections ａnd incubate for 3 days.
E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）.
As a result, the immunostaining should be strongly reduced or even completely prevented. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
CAPN2/牛钙蛋白酶2催化亚基
产品编号：PRO-330
产品规格：2μg/5μg/10μg
Synonyms Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2. Introduction Calpain’s activity is attributed to two main isoforms: ?-calpain ａnd m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes ａnd cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 （?-calpain） or Capn2 （m-calpain）, ａnd a common regulatory subunit encoded by Capn4. Calpain2 （M-calpain） is believed to be membrane bound ａnd functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2. Description CAPN2 consists of an 80 kDa large subunit ａnd a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, ａnd Phenyl-Sepharose CL-4B columns. Source Bovine Myocardium. Physical Appearance Sterile Filtered colorless solution. Formulation 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT ａnd 50% glycerol, pH 7.4. Stability CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Applications This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry （final dilution 1:1＇000）.
B- Add 1μg of protein to 1ml of the diluted antibody solution ａnd mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections ａnd incubate for 3 days.
E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）.
As a result, the immunostaining should be strongly reduced or even completely prevented. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
CAPN2/牛钙蛋白酶2催化亚基
产品编号：PRO-330
产品规格：2μg/5μg/10μg
Synonyms Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2. Introduction Calpain’s activity is attributed to two main isoforms: ?-calpain ａnd m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes ａnd cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 （?-calpain） or Capn2 （m-calpain）, ａnd a common regulatory subunit encoded by Capn4. Calpain2 （M-calpain） is believed to be membrane bound ａnd functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2. Description CAPN2 consists of an 80 kDa large subunit ａnd a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, ａnd Phenyl-Sepharose CL-4B columns. Source Bovine Myocardium. Physical Appearance Sterile Filtered colorless solution. Formulation 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT ａnd 50% glycerol, pH 7.4. Stability CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Applications This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry （final dilution 1:1＇000）.
B- Add 1μg of protein to 1ml of the diluted antibody solution ａnd mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections ａnd incubate for 3 days.
E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）.
As a result, the immunostaining should be strongly reduced or even completely prevented. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
CAPN2/牛钙蛋白酶2催化亚基
产品编号：PRO-330
产品规格：2μg/5μg/10μg
Synonyms Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2. Introduction Calpain’s activity is attributed to two main isoforms: ?-calpain ａnd m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes ａnd cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 （?-calpain） or Capn2 （m-calpain）, ａnd a common regulatory subunit encoded by Capn4. Calpain2 （M-calpain） is believed to be membrane bound ａnd functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2. Description CAPN2 consists of an 80 kDa large subunit ａnd a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, ａnd Phenyl-Sepharose CL-4B columns. Source Bovine Myocardium. Physical Appearance Sterile Filtered colorless solution. Formulation 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT ａnd 50% glycerol, pH 7.4. Stability CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Applications This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry （final dilution 1:1＇000）.
B- Add 1μg of protein to 1ml of the diluted antibody solution ａnd mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections ａnd incubate for 3 days.
E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）.
As a result, the immunostaining should be strongly reduced or even completely prevented. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
CAPN2/牛钙蛋白酶2催化亚基
产品编号：PRO-330
产品规格：2μg/5μg/10μg
产品规格：2μg/5μg/10μg
| Synonyms | Calpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2. |
| Introduction | Calpain’s activity is attributed to two main isoforms: ?-calpain ａnd m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes ａnd cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 （?-calpain） or Capn2 （m-calpain）, ａnd a common regulatory subunit encoded by Capn4. Calpain2 （M-calpain） is believed to be membrane bound ａnd functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2. |
| Description | CAPN2 consists of an 80 kDa large subunit ａnd a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, ａnd Phenyl-Sepharose CL-4B columns. |
| Source | Bovine Myocardium. |
| Physical Appearance | Sterile Filtered colorless solution. |
| Formulation | 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT ａnd 50% glycerol, pH 7.4. |
| Stability | CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Applications | This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection. For adsorption we suggest the following procedureA- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry （final dilution 1:1＇000）. B- Add 1μg of protein to 1ml of the diluted antibody solution ａnd mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections ａnd incubate for 3 days. E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）. As a result, the immunostaining should be strongly reduced or even completely prevented. |
| Usage | Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
