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PRO-445 U1snRNP-68 Human/重组人U1snRNP-68 kDa
点击次数：227发布时间：2012/7/24 16:04:21
更新日期：2024/10/16 15:58:55
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厦门慧嘉生物专业销售PRO-445 U1snRNP-68 Human/重组人U1snRNP-68 kDa剂 Santa Abcam Cst jackson Pierce Sigma Amresco Qiagen Cayman abnova millipore invitrogen merk ebioscience prospec peprotech细胞因子特价。等生物试剂产品。实验为大，诚信经营，为客户提供“质量的产品”和“质的服务"QQ: 1193953234 1048735792 或登陆（向客服人员索取原版说明书） http://www.biohj.com 欢迎广大老师来询！
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
U1snRNP-68 Human/重组人U1snRNP-68 kDa
产品编号：PRO-445
产品规格：5μg/20μg/1mg
Synonyms U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. Introduction Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. Description Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag.
The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered clear solution. Formulation U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Coating concentration 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Purity Greater than 90% as determined by SDS-PAGE. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
产品规格：5μg/20μg/1mg
| Synonyms | U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, snRNP70, U1-70K, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1, U1AP, U170K, U1RNP. |
| Introduction | Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus ａnd consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins （B/B＇, D1, D2, D3, E, F, G） form a core subparticle that is common to all U-snRNP complexes. Both the U1-specific proteins ａnd the Sm core particle are targets of autoantibodies which classically have been called the RNP ａnd RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity ａnd specificity ａnd is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay. Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） ａnd 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. |
| Description | Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein （lacking 66 internal amino acids outside the known epitope-containing areas） fused to a hexahistidine purification tag. The Calculated molecular weight is 44.8 kDa （U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight ａnd the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis）. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered clear solution. |
| Formulation | U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA ａnd 0.02% SDS. |
| Storage Conditions | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Coating concentration | 0.3-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）. Suitable for biotinylation ａnd iodination. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Immunological functions | 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. |
| Applications | Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera （Mixed Connective Tissue Disease）. |
| Usage | Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
