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厦门慧嘉生物专业销售PRO-582 SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸 Santa Abcam Cst jackson Pierce Sigma Amresco Qiagen Cayman abnova millipore invitrogen merk ebioscience prospec peprotech细胞因子特价。等生物试剂产品。实验为大，诚信经营，为客户提供“质量的产品”和“质的服务"QQ: 1193953234 1048735792 或登陆（向客服人员索取原版说明书） http://www.biohj.com 欢迎广大老师来询！
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
Synonyms Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. Introduction SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. Description Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa.
The BM40 is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. Solubility It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD
DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP
APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK
YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI
HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY
LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK
DIDKDLVI. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SPARC Human/重组人酸性分泌蛋白，富含半胱氨酸
产品编号：PRO-582
产品规格：10μg/50μg/1mg
产品规格：10μg/50μg/1mg
| Synonyms | Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic ａnd Rich in Cysteine. |
| Introduction | SPARC, an acronym for "secreted protein, acidic ａnd rich in cysteine", is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, ａnd a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets ａnd macrophages, especially in areas of tissue morphogenesis ａnd remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading ａnd proliferation, ａnd promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions ａnd binds ａnd sequesters PDGF ａnd VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation ａnd inhibits adipogenesis. |
| Description | Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids ａnd having a molecular mass of 34 kDa. The BM40 is purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. |
| Solubility | It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Osteonectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BM-40 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. |
| Purity | Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. |
| Amino acid sequence | MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK DIDKDLVI. |
| Usage | Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
