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Prospec PRO-539 LBP Mouse/重组小鼠脂多糖结合蛋白
点击次数：218发布时间：2012/8/8 17:03:51
更新日期：2024/10/16 15:58:55
所 在 地：美洲
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厦门慧嘉生物专业销售Prospec PRO-539 LBP Mouse/重组小鼠脂多糖结合蛋白 ASSAY ImmunoWay Santa Abcam Cst jackson Pierce Sigma Amresco Qiagen Cayman abnova millipore invitrogen merk ebioscience prospec peprotech细胞因子特价。等生物试剂产品。实验为大，诚信经营，为客户提供“质量的产品”和“质的服务"QQ: 1193953234 1048735792 或登陆（向客服人员索取原版说明书） http://www.biohj.com 欢迎广大老师来询！
LBP Mouse/重组小鼠脂多糖结合蛋白
产品编号：PRO-539
产品规格：2μg/5μg/10μg
Synonyms Lipopolysaccharide-binding protein, LBP, Ly88. Introduction Lipopolysaccharides （LPS） are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein （aka LBP） is a plasma protein which facilitates the diffusion of bacterial LPS （endotoxin）. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein （BPI）, LBP binds LPS ａnd interacts with the CD14 receptor, most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally ａnd functionally related proteins, including BPI, plasma cholesteryl ester transfer protein （CETP）, ａnd phospholipid transfer protein （PLTP）. The LBP gene is found on chromosome 20, directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aggregates to HDL particles, to phospholipid bilayers, ａnd to a binding site on soluble CD14 （sCD14）. sCD14 is capable of speeding up the transfer by receiving an LPS monomer from an LPS aggregate, ａnd then yielding it to an HDL particle, therefore acting as a soluble "shuttle" for an insoluble lipid. Description LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before tranfection the complete mouse LBP cDNA was amplified by PCR ａnd cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon ａnd controlled by SDS page. Attention: His-tag at the c-terminal end of the LBP has no protease site ａnd is not to split off. up to 2μg/ml LBP mediates binding of FITC-LPS （0.5μg/ml） to CD14+CHO transfectants （2 x 1,000,000/ml）. Source Chinese Hamster Ovarian Cells （CHO）. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Recombinant Mouse LBP was lyophilized from a protein solution （1 mg/ml） containing phosphate-buffered saline, pH 7.2. Solubility It is recommended to reconstitute the lyophilized LBP in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized LBP Mouse Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
Please prevent freeze-thaw cycles. Applications ? ELISA ? Western Blotting. characterization On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
LBP Mouse/重组小鼠脂多糖结合蛋白
产品编号：PRO-539
产品规格：2μg/5μg/10μg
Synonyms Lipopolysaccharide-binding protein, LBP, Ly88. Introduction Lipopolysaccharides （LPS） are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein （aka LBP） is a plasma protein which facilitates the diffusion of bacterial LPS （endotoxin）. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein （BPI）, LBP binds LPS ａnd interacts with the CD14 receptor, most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally ａnd functionally related proteins, including BPI, plasma cholesteryl ester transfer protein （CETP）, ａnd phospholipid transfer protein （PLTP）. The LBP gene is found on chromosome 20, directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aggregates to HDL particles, to phospholipid bilayers, ａnd to a binding site on soluble CD14 （sCD14）. sCD14 is capable of speeding up the transfer by receiving an LPS monomer from an LPS aggregate, ａnd then yielding it to an HDL particle, therefore acting as a soluble "shuttle" for an insoluble lipid. Description LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before tranfection the complete mouse LBP cDNA was amplified by PCR ａnd cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon ａnd controlled by SDS page. Attention: His-tag at the c-terminal end of the LBP has no protease site ａnd is not to split off. up to 2μg/ml LBP mediates binding of FITC-LPS （0.5μg/ml） to CD14+CHO transfectants （2 x 1,000,000/ml）. Source Chinese Hamster Ovarian Cells （CHO）. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Recombinant Mouse LBP was lyophilized from a protein solution （1 mg/ml） containing phosphate-buffered saline, pH 7.2. Solubility It is recommended to reconstitute the lyophilized LBP in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized LBP Mouse Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
Please prevent freeze-thaw cycles. Applications ? ELISA ? Western Blotting. characterization On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
LBP Mouse/重组小鼠脂多糖结合蛋白
产品编号：PRO-539
产品规格：2μg/5μg/10μg
Synonyms Lipopolysaccharide-binding protein, LBP, Ly88. Introduction Lipopolysaccharides （LPS） are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein （aka LBP） is a plasma protein which facilitates the diffusion of bacterial LPS （endotoxin）. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein （BPI）, LBP binds LPS ａnd interacts with the CD14 receptor, most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally ａnd functionally related proteins, including BPI, plasma cholesteryl ester transfer protein （CETP）, ａnd phospholipid transfer protein （PLTP）. The LBP gene is found on chromosome 20, directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aggregates to HDL particles, to phospholipid bilayers, ａnd to a binding site on soluble CD14 （sCD14）. sCD14 is capable of speeding up the transfer by receiving an LPS monomer from an LPS aggregate, ａnd then yielding it to an HDL particle, therefore acting as a soluble "shuttle" for an insoluble lipid. Description LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before tranfection the complete mouse LBP cDNA was amplified by PCR ａnd cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon ａnd controlled by SDS page. Attention: His-tag at the c-terminal end of the LBP has no protease site ａnd is not to split off. up to 2μg/ml LBP mediates binding of FITC-LPS （0.5μg/ml） to CD14+CHO transfectants （2 x 1,000,000/ml）. Source Chinese Hamster Ovarian Cells （CHO）. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Recombinant Mouse LBP was lyophilized from a protein solution （1 mg/ml） containing phosphate-buffered saline, pH 7.2. Solubility It is recommended to reconstitute the lyophilized LBP in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized LBP Mouse Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
Please prevent freeze-thaw cycles. Applications ? ELISA ? Western Blotting. characterization On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
LBP Mouse/重组小鼠脂多糖结合蛋白
产品编号：PRO-539
产品规格：2μg/5μg/10μg
Synonyms Lipopolysaccharide-binding protein, LBP, Ly88. Introduction Lipopolysaccharides （LPS） are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein （aka LBP） is a plasma protein which facilitates the diffusion of bacterial LPS （endotoxin）. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein （BPI）, LBP binds LPS ａnd interacts with the CD14 receptor, most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally ａnd functionally related proteins, including BPI, plasma cholesteryl ester transfer protein （CETP）, ａnd phospholipid transfer protein （PLTP）. The LBP gene is found on chromosome 20, directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aggregates to HDL particles, to phospholipid bilayers, ａnd to a binding site on soluble CD14 （sCD14）. sCD14 is capable of speeding up the transfer by receiving an LPS monomer from an LPS aggregate, ａnd then yielding it to an HDL particle, therefore acting as a soluble "shuttle" for an insoluble lipid. Description LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before tranfection the complete mouse LBP cDNA was amplified by PCR ａnd cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon ａnd controlled by SDS page. Attention: His-tag at the c-terminal end of the LBP has no protease site ａnd is not to split off. up to 2μg/ml LBP mediates binding of FITC-LPS （0.5μg/ml） to CD14+CHO transfectants （2 x 1,000,000/ml）. Source Chinese Hamster Ovarian Cells （CHO）. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Recombinant Mouse LBP was lyophilized from a protein solution （1 mg/ml） containing phosphate-buffered saline, pH 7.2. Solubility It is recommended to reconstitute the lyophilized LBP in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized LBP Mouse Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
Please prevent freeze-thaw cycles. Applications ? ELISA ? Western Blotting. characterization On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
LBP Mouse/重组小鼠脂多糖结合蛋白
产品编号：PRO-539
产品规格：2μg/5μg/10μg
Synonyms Lipopolysaccharide-binding protein, LBP, Ly88. Introduction Lipopolysaccharides （LPS） are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein （aka LBP） is a plasma protein which facilitates the diffusion of bacterial LPS （endotoxin）. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein （BPI）, LBP binds LPS ａnd interacts with the CD14 receptor, most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally ａnd functionally related proteins, including BPI, plasma cholesteryl ester transfer protein （CETP）, ａnd phospholipid transfer protein （PLTP）. The LBP gene is found on chromosome 20, directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aggregates to HDL particles, to phospholipid bilayers, ａnd to a binding site on soluble CD14 （sCD14）. sCD14 is capable of speeding up the transfer by receiving an LPS monomer from an LPS aggregate, ａnd then yielding it to an HDL particle, therefore acting as a soluble "shuttle" for an insoluble lipid. Description LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before tranfection the complete mouse LBP cDNA was amplified by PCR ａnd cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon ａnd controlled by SDS page. Attention: His-tag at the c-terminal end of the LBP has no protease site ａnd is not to split off. up to 2μg/ml LBP mediates binding of FITC-LPS （0.5μg/ml） to CD14+CHO transfectants （2 x 1,000,000/ml）. Source Chinese Hamster Ovarian Cells （CHO）. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Recombinant Mouse LBP was lyophilized from a protein solution （1 mg/ml） containing phosphate-buffered saline, pH 7.2. Solubility It is recommended to reconstitute the lyophilized LBP in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized LBP Mouse Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
Please prevent freeze-thaw cycles. Applications ? ELISA ? Western Blotting. characterization On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
LBP Mouse/重组小鼠脂多糖结合蛋白
产品编号：PRO-539
产品规格：2μg/5μg/10μg
Synonyms Lipopolysaccharide-binding protein, LBP, Ly88. Introduction Lipopolysaccharides （LPS） are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein （aka LBP） is a plasma protein which facilitates the diffusion of bacterial LPS （endotoxin）. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein （BPI）, LBP binds LPS ａnd interacts with the CD14 receptor, most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally ａnd functionally related proteins, including BPI, plasma cholesteryl ester transfer protein （CETP）, ａnd phospholipid transfer protein （PLTP）. The LBP gene is found on chromosome 20, directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aggregates to HDL particles, to phospholipid bilayers, ａnd to a binding site on soluble CD14 （sCD14）. sCD14 is capable of speeding up the transfer by receiving an LPS monomer from an LPS aggregate, ａnd then yielding it to an HDL particle, therefore acting as a soluble "shuttle" for an insoluble lipid. Description LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before tranfection the complete mouse LBP cDNA was amplified by PCR ａnd cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon ａnd controlled by SDS page. Attention: His-tag at the c-terminal end of the LBP has no protease site ａnd is not to split off. up to 2μg/ml LBP mediates binding of FITC-LPS （0.5μg/ml） to CD14+CHO transfectants （2 x 1,000,000/ml）. Source Chinese Hamster Ovarian Cells （CHO）. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Recombinant Mouse LBP was lyophilized from a protein solution （1 mg/ml） containing phosphate-buffered saline, pH 7.2. Solubility It is recommended to reconstitute the lyophilized LBP in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized LBP Mouse Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
Please prevent freeze-thaw cycles. Applications ? ELISA ? Western Blotting. characterization On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
LBP Mouse/重组小鼠脂多糖结合蛋白
产品编号：PRO-539
产品规格：2μg/5μg/10μg
Synonyms Lipopolysaccharide-binding protein, LBP, Ly88. Introduction Lipopolysaccharides （LPS） are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein （aka LBP） is a plasma protein which facilitates the diffusion of bacterial LPS （endotoxin）. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein （BPI）, LBP binds LPS ａnd interacts with the CD14 receptor, most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally ａnd functionally related proteins, including BPI, plasma cholesteryl ester transfer protein （CETP）, ａnd phospholipid transfer protein （PLTP）. The LBP gene is found on chromosome 20, directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aggregates to HDL particles, to phospholipid bilayers, ａnd to a binding site on soluble CD14 （sCD14）. sCD14 is capable of speeding up the transfer by receiving an LPS monomer from an LPS aggregate, ａnd then yielding it to an HDL particle, therefore acting as a soluble "shuttle" for an insoluble lipid. Description LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before tranfection the complete mouse LBP cDNA was amplified by PCR ａnd cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon ａnd controlled by SDS page. Attention: His-tag at the c-terminal end of the LBP has no protease site ａnd is not to split off. up to 2μg/ml LBP mediates binding of FITC-LPS （0.5μg/ml） to CD14+CHO transfectants （2 x 1,000,000/ml）. Source Chinese Hamster Ovarian Cells （CHO）. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Recombinant Mouse LBP was lyophilized from a protein solution （1 mg/ml） containing phosphate-buffered saline, pH 7.2. Solubility It is recommended to reconstitute the lyophilized LBP in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized LBP Mouse Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
Please prevent freeze-thaw cycles. Applications ? ELISA ? Western Blotting. characterization On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
LBP Mouse/重组小鼠脂多糖结合蛋白
产品编号：PRO-539
产品规格：2μg/5μg/10μg
Synonyms Lipopolysaccharide-binding protein, LBP, Ly88. Introduction Lipopolysaccharides （LPS） are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein （aka LBP） is a plasma protein which facilitates the diffusion of bacterial LPS （endotoxin）. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein （BPI）, LBP binds LPS ａnd interacts with the CD14 receptor, most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally ａnd functionally related proteins, including BPI, plasma cholesteryl ester transfer protein （CETP）, ａnd phospholipid transfer protein （PLTP）. The LBP gene is found on chromosome 20, directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aggregates to HDL particles, to phospholipid bilayers, ａnd to a binding site on soluble CD14 （sCD14）. sCD14 is capable of speeding up the transfer by receiving an LPS monomer from an LPS aggregate, ａnd then yielding it to an HDL particle, therefore acting as a soluble "shuttle" for an insoluble lipid. Description LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before tranfection the complete mouse LBP cDNA was amplified by PCR ａnd cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon ａnd controlled by SDS page. Attention: His-tag at the c-terminal end of the LBP has no protease site ａnd is not to split off. up to 2μg/ml LBP mediates binding of FITC-LPS （0.5μg/ml） to CD14+CHO transfectants （2 x 1,000,000/ml）. Source Chinese Hamster Ovarian Cells （CHO）. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Recombinant Mouse LBP was lyophilized from a protein solution （1 mg/ml） containing phosphate-buffered saline, pH 7.2. Solubility It is recommended to reconstitute the lyophilized LBP in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized LBP Mouse Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
Please prevent freeze-thaw cycles. Applications ? ELISA ? Western Blotting. characterization On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
产品规格：2μg/5μg/10μg
| Synonyms | Lipopolysaccharide-binding protein, LBP, Ly88. |
| Introduction | Lipopolysaccharides （LPS） are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein （aka LBP） is a plasma protein which facilitates the diffusion of bacterial LPS （endotoxin）. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein （BPI）, LBP binds LPS ａnd interacts with the CD14 receptor, most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally ａnd functionally related proteins, including BPI, plasma cholesteryl ester transfer protein （CETP）, ａnd phospholipid transfer protein （PLTP）. The LBP gene is found on chromosome 20, directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aggregates to HDL particles, to phospholipid bilayers, ａnd to a binding site on soluble CD14 （sCD14）. sCD14 is capable of speeding up the transfer by receiving an LPS monomer from an LPS aggregate, ａnd then yielding it to an HDL particle, therefore acting as a soluble "shuttle" for an insoluble lipid. |
| Description | LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before tranfection the complete mouse LBP cDNA was amplified by PCR ａnd cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon ａnd controlled by SDS page. Attention: His-tag at the c-terminal end of the LBP has no protease site ａnd is not to split off. up to 2μg/ml LBP mediates binding of FITC-LPS （0.5μg/ml） to CD14+CHO transfectants （2 x 1,000,000/ml）. |
| Source | Chinese Hamster Ovarian Cells （CHO）. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | Recombinant Mouse LBP was lyophilized from a protein solution （1 mg/ml） containing phosphate-buffered saline, pH 7.2. |
| Solubility | It is recommended to reconstitute the lyophilized LBP in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized LBP Mouse Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days ａnd for future use below -18°C. Please prevent freeze-thaw cycles. |
| Applications | ? ELISA ? Western Blotting. |
| characterization | On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa. |
| Usage | Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
