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厦门慧嘉生物专业销售Prospec PRO-397 Ku P70/P80 Human/ 重组人Ku P70/P80 ASSAY ImmunoWay Santa Abcam Cst jackson Pierce Sigma Amresco Qiagen Cayman abnova millipore invitrogen merk ebioscience prospec peprotech细胞因子特价。等生物试剂产品。实验为大，诚信经营，为客户提供“质量的产品”和“质的服务"QQ: 1193953234 1048735792 或登陆（向客服人员索取原版说明书） http://www.biohj.com 欢迎广大老师来询！
Ku P70/P80 Human/ 重组人Ku P70/P80
产品编号：PRO-397
产品规格：5μg/20μg/1mg
Synonyms ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, Lupus Ku autoantigen protein p70, Ku70, 70 kDa subunit of Ku antigen, Thyroid-lupus autoantigen, TLAA, CTC box-binding factor 75 kDa subunit, CTCBF, CTC75, DNA-repair protein XRCC6, G22P1, XRCC6, ML8. Introduction Ku antigen consists of two polypeptides （70 kDa ａnd 80 kDa; the p80 subunit is occasionally called p86）. The Ku heterodimer provides DNA-binding ａnd regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V（D）J recombination, telomere maintenance, ａnd the regulation of specific gene transcription.
Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome （up to 20% of patients） but have also been detectable in about 20 % of patients with primary pulmonary hypertension （as a complication of overlap）, in 5-10 % of SLE ａnd MCTD patients, ａnd in up to 20 % of patients with primary Sj?gren＇s syndrome.
In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent. Description KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit ａnd 83,528 Dalton for the p80 subunit.
Ku （P70/P80） is expressed with a -6xHis tag ａnd purified by proprietary chromatographic techniques. Source Sf9 insect cells. Physical Appearance Sterile Filtered clear solution. Formulation KuP70/P80is supplied in16mM HEPES buffer pH-8, 160mM NaCl, ａnd 20% glycerol. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & anti-Ku autoantibody-positive patient sera. Coating concentration 0.2-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Ku P70/P80 Human/ 重组人Ku P70/P80
产品编号：PRO-397
产品规格：5μg/20μg/1mg
Synonyms ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, Lupus Ku autoantigen protein p70, Ku70, 70 kDa subunit of Ku antigen, Thyroid-lupus autoantigen, TLAA, CTC box-binding factor 75 kDa subunit, CTCBF, CTC75, DNA-repair protein XRCC6, G22P1, XRCC6, ML8. Introduction Ku antigen consists of two polypeptides （70 kDa ａnd 80 kDa; the p80 subunit is occasionally called p86）. The Ku heterodimer provides DNA-binding ａnd regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V（D）J recombination, telomere maintenance, ａnd the regulation of specific gene transcription.
Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome （up to 20% of patients） but have also been detectable in about 20 % of patients with primary pulmonary hypertension （as a complication of overlap）, in 5-10 % of SLE ａnd MCTD patients, ａnd in up to 20 % of patients with primary Sj?gren＇s syndrome.
In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent. Description KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit ａnd 83,528 Dalton for the p80 subunit.
Ku （P70/P80） is expressed with a -6xHis tag ａnd purified by proprietary chromatographic techniques. Source Sf9 insect cells. Physical Appearance Sterile Filtered clear solution. Formulation KuP70/P80is supplied in16mM HEPES buffer pH-8, 160mM NaCl, ａnd 20% glycerol. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & anti-Ku autoantibody-positive patient sera. Coating concentration 0.2-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Ku P70/P80 Human/ 重组人Ku P70/P80
产品编号：PRO-397
产品规格：5μg/20μg/1mg
Synonyms ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, Lupus Ku autoantigen protein p70, Ku70, 70 kDa subunit of Ku antigen, Thyroid-lupus autoantigen, TLAA, CTC box-binding factor 75 kDa subunit, CTCBF, CTC75, DNA-repair protein XRCC6, G22P1, XRCC6, ML8. Introduction Ku antigen consists of two polypeptides （70 kDa ａnd 80 kDa; the p80 subunit is occasionally called p86）. The Ku heterodimer provides DNA-binding ａnd regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V（D）J recombination, telomere maintenance, ａnd the regulation of specific gene transcription.
Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome （up to 20% of patients） but have also been detectable in about 20 % of patients with primary pulmonary hypertension （as a complication of overlap）, in 5-10 % of SLE ａnd MCTD patients, ａnd in up to 20 % of patients with primary Sj?gren＇s syndrome.
In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent. Description KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit ａnd 83,528 Dalton for the p80 subunit.
Ku （P70/P80） is expressed with a -6xHis tag ａnd purified by proprietary chromatographic techniques. Source Sf9 insect cells. Physical Appearance Sterile Filtered clear solution. Formulation KuP70/P80is supplied in16mM HEPES buffer pH-8, 160mM NaCl, ａnd 20% glycerol. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & anti-Ku autoantibody-positive patient sera. Coating concentration 0.2-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Ku P70/P80 Human/ 重组人Ku P70/P80
产品编号：PRO-397
产品规格：5μg/20μg/1mg
Synonyms ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, Lupus Ku autoantigen protein p70, Ku70, 70 kDa subunit of Ku antigen, Thyroid-lupus autoantigen, TLAA, CTC box-binding factor 75 kDa subunit, CTCBF, CTC75, DNA-repair protein XRCC6, G22P1, XRCC6, ML8. Introduction Ku antigen consists of two polypeptides （70 kDa ａnd 80 kDa; the p80 subunit is occasionally called p86）. The Ku heterodimer provides DNA-binding ａnd regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V（D）J recombination, telomere maintenance, ａnd the regulation of specific gene transcription.
Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome （up to 20% of patients） but have also been detectable in about 20 % of patients with primary pulmonary hypertension （as a complication of overlap）, in 5-10 % of SLE ａnd MCTD patients, ａnd in up to 20 % of patients with primary Sj?gren＇s syndrome.
In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent. Description KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit ａnd 83,528 Dalton for the p80 subunit.
Ku （P70/P80） is expressed with a -6xHis tag ａnd purified by proprietary chromatographic techniques. Source Sf9 insect cells. Physical Appearance Sterile Filtered clear solution. Formulation KuP70/P80is supplied in16mM HEPES buffer pH-8, 160mM NaCl, ａnd 20% glycerol. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & anti-Ku autoantibody-positive patient sera. Coating concentration 0.2-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Ku P70/P80 Human/ 重组人Ku P70/P80
产品编号：PRO-397
产品规格：5μg/20μg/1mg
Synonyms ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, Lupus Ku autoantigen protein p70, Ku70, 70 kDa subunit of Ku antigen, Thyroid-lupus autoantigen, TLAA, CTC box-binding factor 75 kDa subunit, CTCBF, CTC75, DNA-repair protein XRCC6, G22P1, XRCC6, ML8. Introduction Ku antigen consists of two polypeptides （70 kDa ａnd 80 kDa; the p80 subunit is occasionally called p86）. The Ku heterodimer provides DNA-binding ａnd regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V（D）J recombination, telomere maintenance, ａnd the regulation of specific gene transcription.
Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome （up to 20% of patients） but have also been detectable in about 20 % of patients with primary pulmonary hypertension （as a complication of overlap）, in 5-10 % of SLE ａnd MCTD patients, ａnd in up to 20 % of patients with primary Sj?gren＇s syndrome.
In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent. Description KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit ａnd 83,528 Dalton for the p80 subunit.
Ku （P70/P80） is expressed with a -6xHis tag ａnd purified by proprietary chromatographic techniques. Source Sf9 insect cells. Physical Appearance Sterile Filtered clear solution. Formulation KuP70/P80is supplied in16mM HEPES buffer pH-8, 160mM NaCl, ａnd 20% glycerol. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & anti-Ku autoantibody-positive patient sera. Coating concentration 0.2-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Ku P70/P80 Human/ 重组人Ku P70/P80
产品编号：PRO-397
产品规格：5μg/20μg/1mg
Synonyms ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, Lupus Ku autoantigen protein p70, Ku70, 70 kDa subunit of Ku antigen, Thyroid-lupus autoantigen, TLAA, CTC box-binding factor 75 kDa subunit, CTCBF, CTC75, DNA-repair protein XRCC6, G22P1, XRCC6, ML8. Introduction Ku antigen consists of two polypeptides （70 kDa ａnd 80 kDa; the p80 subunit is occasionally called p86）. The Ku heterodimer provides DNA-binding ａnd regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V（D）J recombination, telomere maintenance, ａnd the regulation of specific gene transcription.
Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome （up to 20% of patients） but have also been detectable in about 20 % of patients with primary pulmonary hypertension （as a complication of overlap）, in 5-10 % of SLE ａnd MCTD patients, ａnd in up to 20 % of patients with primary Sj?gren＇s syndrome.
In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent. Description KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit ａnd 83,528 Dalton for the p80 subunit.
Ku （P70/P80） is expressed with a -6xHis tag ａnd purified by proprietary chromatographic techniques. Source Sf9 insect cells. Physical Appearance Sterile Filtered clear solution. Formulation KuP70/P80is supplied in16mM HEPES buffer pH-8, 160mM NaCl, ａnd 20% glycerol. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & anti-Ku autoantibody-positive patient sera. Coating concentration 0.2-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Ku P70/P80 Human/ 重组人Ku P70/P80
产品编号：PRO-397
产品规格：5μg/20μg/1mg
Synonyms ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, Lupus Ku autoantigen protein p70, Ku70, 70 kDa subunit of Ku antigen, Thyroid-lupus autoantigen, TLAA, CTC box-binding factor 75 kDa subunit, CTCBF, CTC75, DNA-repair protein XRCC6, G22P1, XRCC6, ML8. Introduction Ku antigen consists of two polypeptides （70 kDa ａnd 80 kDa; the p80 subunit is occasionally called p86）. The Ku heterodimer provides DNA-binding ａnd regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V（D）J recombination, telomere maintenance, ａnd the regulation of specific gene transcription.
Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome （up to 20% of patients） but have also been detectable in about 20 % of patients with primary pulmonary hypertension （as a complication of overlap）, in 5-10 % of SLE ａnd MCTD patients, ａnd in up to 20 % of patients with primary Sj?gren＇s syndrome.
In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent. Description KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit ａnd 83,528 Dalton for the p80 subunit.
Ku （P70/P80） is expressed with a -6xHis tag ａnd purified by proprietary chromatographic techniques. Source Sf9 insect cells. Physical Appearance Sterile Filtered clear solution. Formulation KuP70/P80is supplied in16mM HEPES buffer pH-8, 160mM NaCl, ａnd 20% glycerol. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & anti-Ku autoantibody-positive patient sera. Coating concentration 0.2-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Ku P70/P80 Human/ 重组人Ku P70/P80
产品编号：PRO-397
产品规格：5μg/20μg/1mg
Synonyms ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, Lupus Ku autoantigen protein p70, Ku70, 70 kDa subunit of Ku antigen, Thyroid-lupus autoantigen, TLAA, CTC box-binding factor 75 kDa subunit, CTCBF, CTC75, DNA-repair protein XRCC6, G22P1, XRCC6, ML8. Introduction Ku antigen consists of two polypeptides （70 kDa ａnd 80 kDa; the p80 subunit is occasionally called p86）. The Ku heterodimer provides DNA-binding ａnd regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V（D）J recombination, telomere maintenance, ａnd the regulation of specific gene transcription.
Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome （up to 20% of patients） but have also been detectable in about 20 % of patients with primary pulmonary hypertension （as a complication of overlap）, in 5-10 % of SLE ａnd MCTD patients, ａnd in up to 20 % of patients with primary Sj?gren＇s syndrome.
In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent. Description KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit ａnd 83,528 Dalton for the p80 subunit.
Ku （P70/P80） is expressed with a -6xHis tag ａnd purified by proprietary chromatographic techniques. Source Sf9 insect cells. Physical Appearance Sterile Filtered clear solution. Formulation KuP70/P80is supplied in16mM HEPES buffer pH-8, 160mM NaCl, ａnd 20% glycerol. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & anti-Ku autoantibody-positive patient sera. Coating concentration 0.2-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Ku P70/P80 Human/ 重组人Ku P70/P80
产品编号：PRO-397
产品规格：5μg/20μg/1mg
Synonyms ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, Lupus Ku autoantigen protein p70, Ku70, 70 kDa subunit of Ku antigen, Thyroid-lupus autoantigen, TLAA, CTC box-binding factor 75 kDa subunit, CTCBF, CTC75, DNA-repair protein XRCC6, G22P1, XRCC6, ML8. Introduction Ku antigen consists of two polypeptides （70 kDa ａnd 80 kDa; the p80 subunit is occasionally called p86）. The Ku heterodimer provides DNA-binding ａnd regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V（D）J recombination, telomere maintenance, ａnd the regulation of specific gene transcription.
Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome （up to 20% of patients） but have also been detectable in about 20 % of patients with primary pulmonary hypertension （as a complication of overlap）, in 5-10 % of SLE ａnd MCTD patients, ａnd in up to 20 % of patients with primary Sj?gren＇s syndrome.
In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent. Description KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit ａnd 83,528 Dalton for the p80 subunit.
Ku （P70/P80） is expressed with a -6xHis tag ａnd purified by proprietary chromatographic techniques. Source Sf9 insect cells. Physical Appearance Sterile Filtered clear solution. Formulation KuP70/P80is supplied in16mM HEPES buffer pH-8, 160mM NaCl, ａnd 20% glycerol. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & anti-Ku autoantibody-positive patient sera. Coating concentration 0.2-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Ku P70/P80 Human/ 重组人Ku P70/P80
产品编号：PRO-397
产品规格：5μg/20μg/1mg
Synonyms ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, Lupus Ku autoantigen protein p70, Ku70, 70 kDa subunit of Ku antigen, Thyroid-lupus autoantigen, TLAA, CTC box-binding factor 75 kDa subunit, CTCBF, CTC75, DNA-repair protein XRCC6, G22P1, XRCC6, ML8. Introduction Ku antigen consists of two polypeptides （70 kDa ａnd 80 kDa; the p80 subunit is occasionally called p86）. The Ku heterodimer provides DNA-binding ａnd regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V（D）J recombination, telomere maintenance, ａnd the regulation of specific gene transcription.
Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome （up to 20% of patients） but have also been detectable in about 20 % of patients with primary pulmonary hypertension （as a complication of overlap）, in 5-10 % of SLE ａnd MCTD patients, ａnd in up to 20 % of patients with primary Sj?gren＇s syndrome.
In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent. Description KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit ａnd 83,528 Dalton for the p80 subunit.
Ku （P70/P80） is expressed with a -6xHis tag ａnd purified by proprietary chromatographic techniques. Source Sf9 insect cells. Physical Appearance Sterile Filtered clear solution. Formulation KuP70/P80is supplied in16mM HEPES buffer pH-8, 160mM NaCl, ａnd 20% glycerol. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & anti-Ku autoantibody-positive patient sera. Coating concentration 0.2-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Ku P70/P80 Human/ 重组人Ku P70/P80
产品编号：PRO-397
产品规格：5μg/20μg/1mg
Synonyms ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, Lupus Ku autoantigen protein p70, Ku70, 70 kDa subunit of Ku antigen, Thyroid-lupus autoantigen, TLAA, CTC box-binding factor 75 kDa subunit, CTCBF, CTC75, DNA-repair protein XRCC6, G22P1, XRCC6, ML8. Introduction Ku antigen consists of two polypeptides （70 kDa ａnd 80 kDa; the p80 subunit is occasionally called p86）. The Ku heterodimer provides DNA-binding ａnd regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V（D）J recombination, telomere maintenance, ａnd the regulation of specific gene transcription.
Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome （up to 20% of patients） but have also been detectable in about 20 % of patients with primary pulmonary hypertension （as a complication of overlap）, in 5-10 % of SLE ａnd MCTD patients, ａnd in up to 20 % of patients with primary Sj?gren＇s syndrome.
In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent. Description KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit ａnd 83,528 Dalton for the p80 subunit.
Ku （P70/P80） is expressed with a -6xHis tag ａnd purified by proprietary chromatographic techniques. Source Sf9 insect cells. Physical Appearance Sterile Filtered clear solution. Formulation KuP70/P80is supplied in16mM HEPES buffer pH-8, 160mM NaCl, ａnd 20% glycerol. Immunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. Applications Western-Blot with monoclonal anti-hexa-His-tag antibody & anti-Ku autoantibody-positive patient sera. Coating concentration 0.2-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）.
Suitable for biotinylation ａnd iodination. Storage Conditions Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles. Purity Greater than 90% as determined by SDS-PAGE. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
产品规格：5μg/20μg/1mg
| Synonyms | ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, Lupus Ku autoantigen protein p70, Ku70, 70 kDa subunit of Ku antigen, Thyroid-lupus autoantigen, TLAA, CTC box-binding factor 75 kDa subunit, CTCBF, CTC75, DNA-repair protein XRCC6, G22P1, XRCC6, ML8. |
| Introduction | Ku antigen consists of two polypeptides （70 kDa ａnd 80 kDa; the p80 subunit is occasionally called p86）. The Ku heterodimer provides DNA-binding ａnd regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V（D）J recombination, telomere maintenance, ａnd the regulation of specific gene transcription. Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome （up to 20% of patients） but have also been detectable in about 20 % of patients with primary pulmonary hypertension （as a complication of overlap）, in 5-10 % of SLE ａnd MCTD patients, ａnd in up to 20 % of patients with primary Sj?gren＇s syndrome. In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent. |
| Description | KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit ａnd 83,528 Dalton for the p80 subunit. Ku （P70/P80） is expressed with a -6xHis tag ａnd purified by proprietary chromatographic techniques. |
| Source | Sf9 insect cells. |
| Physical Appearance | Sterile Filtered clear solution. |
| Formulation | KuP70/P80is supplied in16mM HEPES buffer pH-8, 160mM NaCl, ａnd 20% glycerol. |
| Immunological functions | 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test （checker-board analysis of positive/negative sera panels including CDC international reference sera）. |
| Applications | Western-Blot with monoclonal anti-hexa-His-tag antibody & anti-Ku autoantibody-positive patient sera. |
| Coating concentration | 0.2-0.6 μg/ml （depending on the type of ELISA plate ａnd coating buffer）. Suitable for biotinylation ａnd iodination. |
| Storage Conditions | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Usage | Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
