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厦门慧嘉生物专业销售Prospec PRO-362 Hirudin/重组水蛭素 ASSAY ImmunoWay Santa Abcam Cst jackson Pierce Sigma Amresco Qiagen Cayman abnova millipore invitrogen merk ebioscience prospec peprotech细胞因子特价。等生物试剂产品。实验为大，诚信经营，为客户提供“质量的产品”和“质的服务"QQ: 1193953234 1048735792 或登陆（向客服人员索取原版说明书） http://www.biohj.com 欢迎广大老师来询！
Hirudin/重组水蛭素
产品编号：PRO-362
产品规格：2μg/10μg/1mg
Introduction Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech unlike heparin. Hirudin acts directly on thrombin rather than through other clotting factors. The mechanism of Hirudin-thrombin appears to be unique. The conversion of fibrinogen into fibrin by the serine protease enzyme thrombin is a major event in the final stages of blood coagulation. In the final stages of coagulation prothrombinase converts prothrombin into thrombin. Fibrin is subsequently cross linked by factor XIII to form a blood clot. The primary inhibitor of thrombin in normal blood circulation is antithrombin III. The anticoagulatant activity of hirudin is derived from its ability to inhibit the pro-coagulant activity of thrombin （similar to antithrombin III activity）. Hirudin is the strongest natural inhibitor of thrombin. Hirudin binds to ａnd inhibits only the activity of thrombin forms with a specific activity on fibrinogen contrasting to antithrombin III activity. Therefore, hirudin has a thrombolytic activity since it prevents or dissolves the formation of clots ａnd thrombi. Hirudin also has therapeutic significance in blood coagulation disorders, in the treatment of skin hematomas ａnd of superficial varicose veins. Hirudin does not hinder with the biological activity of other serum proteins ａnd can also act on complexed thrombin, thus having an advantage over more common anticoagulants ａnd thrombolytics like heparin for example. It is complicated to extract large quantities of hirudin from natural sources; therefore a method for producing ａnd purifying hirudin using recombinant biotechnology has been developed. Description Recombinant Hirudin is derived from yeast ａnd the polypeptide chain contains 65 amino acids ａnd its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule ａnd the absence of a sulfate group on the tyrosine at position 63.
The Recombinant Hirudin is purified by proprietary chromatographic techniques. Source Pichia Pastoris. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 ａnd 2% mannitol. Solubility It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Hirudin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Biological Activity The bilogical activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Hirudin/重组水蛭素
产品编号：PRO-362
产品规格：2μg/10μg/1mg
Introduction Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech unlike heparin. Hirudin acts directly on thrombin rather than through other clotting factors. The mechanism of Hirudin-thrombin appears to be unique. The conversion of fibrinogen into fibrin by the serine protease enzyme thrombin is a major event in the final stages of blood coagulation. In the final stages of coagulation prothrombinase converts prothrombin into thrombin. Fibrin is subsequently cross linked by factor XIII to form a blood clot. The primary inhibitor of thrombin in normal blood circulation is antithrombin III. The anticoagulatant activity of hirudin is derived from its ability to inhibit the pro-coagulant activity of thrombin （similar to antithrombin III activity）. Hirudin is the strongest natural inhibitor of thrombin. Hirudin binds to ａnd inhibits only the activity of thrombin forms with a specific activity on fibrinogen contrasting to antithrombin III activity. Therefore, hirudin has a thrombolytic activity since it prevents or dissolves the formation of clots ａnd thrombi. Hirudin also has therapeutic significance in blood coagulation disorders, in the treatment of skin hematomas ａnd of superficial varicose veins. Hirudin does not hinder with the biological activity of other serum proteins ａnd can also act on complexed thrombin, thus having an advantage over more common anticoagulants ａnd thrombolytics like heparin for example. It is complicated to extract large quantities of hirudin from natural sources; therefore a method for producing ａnd purifying hirudin using recombinant biotechnology has been developed. Description Recombinant Hirudin is derived from yeast ａnd the polypeptide chain contains 65 amino acids ａnd its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule ａnd the absence of a sulfate group on the tyrosine at position 63.
The Recombinant Hirudin is purified by proprietary chromatographic techniques. Source Pichia Pastoris. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 ａnd 2% mannitol. Solubility It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Hirudin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Biological Activity The bilogical activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Hirudin/重组水蛭素
产品编号：PRO-362
产品规格：2μg/10μg/1mg
Introduction Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech unlike heparin. Hirudin acts directly on thrombin rather than through other clotting factors. The mechanism of Hirudin-thrombin appears to be unique. The conversion of fibrinogen into fibrin by the serine protease enzyme thrombin is a major event in the final stages of blood coagulation. In the final stages of coagulation prothrombinase converts prothrombin into thrombin. Fibrin is subsequently cross linked by factor XIII to form a blood clot. The primary inhibitor of thrombin in normal blood circulation is antithrombin III. The anticoagulatant activity of hirudin is derived from its ability to inhibit the pro-coagulant activity of thrombin （similar to antithrombin III activity）. Hirudin is the strongest natural inhibitor of thrombin. Hirudin binds to ａnd inhibits only the activity of thrombin forms with a specific activity on fibrinogen contrasting to antithrombin III activity. Therefore, hirudin has a thrombolytic activity since it prevents or dissolves the formation of clots ａnd thrombi. Hirudin also has therapeutic significance in blood coagulation disorders, in the treatment of skin hematomas ａnd of superficial varicose veins. Hirudin does not hinder with the biological activity of other serum proteins ａnd can also act on complexed thrombin, thus having an advantage over more common anticoagulants ａnd thrombolytics like heparin for example. It is complicated to extract large quantities of hirudin from natural sources; therefore a method for producing ａnd purifying hirudin using recombinant biotechnology has been developed. Description Recombinant Hirudin is derived from yeast ａnd the polypeptide chain contains 65 amino acids ａnd its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule ａnd the absence of a sulfate group on the tyrosine at position 63.
The Recombinant Hirudin is purified by proprietary chromatographic techniques. Source Pichia Pastoris. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 ａnd 2% mannitol. Solubility It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Hirudin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Biological Activity The bilogical activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Hirudin/重组水蛭素
产品编号：PRO-362
产品规格：2μg/10μg/1mg
Introduction Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech unlike heparin. Hirudin acts directly on thrombin rather than through other clotting factors. The mechanism of Hirudin-thrombin appears to be unique. The conversion of fibrinogen into fibrin by the serine protease enzyme thrombin is a major event in the final stages of blood coagulation. In the final stages of coagulation prothrombinase converts prothrombin into thrombin. Fibrin is subsequently cross linked by factor XIII to form a blood clot. The primary inhibitor of thrombin in normal blood circulation is antithrombin III. The anticoagulatant activity of hirudin is derived from its ability to inhibit the pro-coagulant activity of thrombin （similar to antithrombin III activity）. Hirudin is the strongest natural inhibitor of thrombin. Hirudin binds to ａnd inhibits only the activity of thrombin forms with a specific activity on fibrinogen contrasting to antithrombin III activity. Therefore, hirudin has a thrombolytic activity since it prevents or dissolves the formation of clots ａnd thrombi. Hirudin also has therapeutic significance in blood coagulation disorders, in the treatment of skin hematomas ａnd of superficial varicose veins. Hirudin does not hinder with the biological activity of other serum proteins ａnd can also act on complexed thrombin, thus having an advantage over more common anticoagulants ａnd thrombolytics like heparin for example. It is complicated to extract large quantities of hirudin from natural sources; therefore a method for producing ａnd purifying hirudin using recombinant biotechnology has been developed. Description Recombinant Hirudin is derived from yeast ａnd the polypeptide chain contains 65 amino acids ａnd its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule ａnd the absence of a sulfate group on the tyrosine at position 63.
The Recombinant Hirudin is purified by proprietary chromatographic techniques. Source Pichia Pastoris. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 ａnd 2% mannitol. Solubility It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Hirudin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Biological Activity The bilogical activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Hirudin/重组水蛭素
产品编号：PRO-362
产品规格：2μg/10μg/1mg
Introduction Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech unlike heparin. Hirudin acts directly on thrombin rather than through other clotting factors. The mechanism of Hirudin-thrombin appears to be unique. The conversion of fibrinogen into fibrin by the serine protease enzyme thrombin is a major event in the final stages of blood coagulation. In the final stages of coagulation prothrombinase converts prothrombin into thrombin. Fibrin is subsequently cross linked by factor XIII to form a blood clot. The primary inhibitor of thrombin in normal blood circulation is antithrombin III. The anticoagulatant activity of hirudin is derived from its ability to inhibit the pro-coagulant activity of thrombin （similar to antithrombin III activity）. Hirudin is the strongest natural inhibitor of thrombin. Hirudin binds to ａnd inhibits only the activity of thrombin forms with a specific activity on fibrinogen contrasting to antithrombin III activity. Therefore, hirudin has a thrombolytic activity since it prevents or dissolves the formation of clots ａnd thrombi. Hirudin also has therapeutic significance in blood coagulation disorders, in the treatment of skin hematomas ａnd of superficial varicose veins. Hirudin does not hinder with the biological activity of other serum proteins ａnd can also act on complexed thrombin, thus having an advantage over more common anticoagulants ａnd thrombolytics like heparin for example. It is complicated to extract large quantities of hirudin from natural sources; therefore a method for producing ａnd purifying hirudin using recombinant biotechnology has been developed. Description Recombinant Hirudin is derived from yeast ａnd the polypeptide chain contains 65 amino acids ａnd its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule ａnd the absence of a sulfate group on the tyrosine at position 63.
The Recombinant Hirudin is purified by proprietary chromatographic techniques. Source Pichia Pastoris. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 ａnd 2% mannitol. Solubility It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Hirudin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Biological Activity The bilogical activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Hirudin/重组水蛭素
产品编号：PRO-362
产品规格：2μg/10μg/1mg
Introduction Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech unlike heparin. Hirudin acts directly on thrombin rather than through other clotting factors. The mechanism of Hirudin-thrombin appears to be unique. The conversion of fibrinogen into fibrin by the serine protease enzyme thrombin is a major event in the final stages of blood coagulation. In the final stages of coagulation prothrombinase converts prothrombin into thrombin. Fibrin is subsequently cross linked by factor XIII to form a blood clot. The primary inhibitor of thrombin in normal blood circulation is antithrombin III. The anticoagulatant activity of hirudin is derived from its ability to inhibit the pro-coagulant activity of thrombin （similar to antithrombin III activity）. Hirudin is the strongest natural inhibitor of thrombin. Hirudin binds to ａnd inhibits only the activity of thrombin forms with a specific activity on fibrinogen contrasting to antithrombin III activity. Therefore, hirudin has a thrombolytic activity since it prevents or dissolves the formation of clots ａnd thrombi. Hirudin also has therapeutic significance in blood coagulation disorders, in the treatment of skin hematomas ａnd of superficial varicose veins. Hirudin does not hinder with the biological activity of other serum proteins ａnd can also act on complexed thrombin, thus having an advantage over more common anticoagulants ａnd thrombolytics like heparin for example. It is complicated to extract large quantities of hirudin from natural sources; therefore a method for producing ａnd purifying hirudin using recombinant biotechnology has been developed. Description Recombinant Hirudin is derived from yeast ａnd the polypeptide chain contains 65 amino acids ａnd its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule ａnd the absence of a sulfate group on the tyrosine at position 63.
The Recombinant Hirudin is purified by proprietary chromatographic techniques. Source Pichia Pastoris. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 ａnd 2% mannitol. Solubility It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Hirudin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Biological Activity The bilogical activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Hirudin/重组水蛭素
产品编号：PRO-362
产品规格：2μg/10μg/1mg
Introduction Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech unlike heparin. Hirudin acts directly on thrombin rather than through other clotting factors. The mechanism of Hirudin-thrombin appears to be unique. The conversion of fibrinogen into fibrin by the serine protease enzyme thrombin is a major event in the final stages of blood coagulation. In the final stages of coagulation prothrombinase converts prothrombin into thrombin. Fibrin is subsequently cross linked by factor XIII to form a blood clot. The primary inhibitor of thrombin in normal blood circulation is antithrombin III. The anticoagulatant activity of hirudin is derived from its ability to inhibit the pro-coagulant activity of thrombin （similar to antithrombin III activity）. Hirudin is the strongest natural inhibitor of thrombin. Hirudin binds to ａnd inhibits only the activity of thrombin forms with a specific activity on fibrinogen contrasting to antithrombin III activity. Therefore, hirudin has a thrombolytic activity since it prevents or dissolves the formation of clots ａnd thrombi. Hirudin also has therapeutic significance in blood coagulation disorders, in the treatment of skin hematomas ａnd of superficial varicose veins. Hirudin does not hinder with the biological activity of other serum proteins ａnd can also act on complexed thrombin, thus having an advantage over more common anticoagulants ａnd thrombolytics like heparin for example. It is complicated to extract large quantities of hirudin from natural sources; therefore a method for producing ａnd purifying hirudin using recombinant biotechnology has been developed. Description Recombinant Hirudin is derived from yeast ａnd the polypeptide chain contains 65 amino acids ａnd its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule ａnd the absence of a sulfate group on the tyrosine at position 63.
The Recombinant Hirudin is purified by proprietary chromatographic techniques. Source Pichia Pastoris. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 ａnd 2% mannitol. Solubility It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Hirudin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Biological Activity The bilogical activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Hirudin/重组水蛭素
产品编号：PRO-362
产品规格：2μg/10μg/1mg
Introduction Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech unlike heparin. Hirudin acts directly on thrombin rather than through other clotting factors. The mechanism of Hirudin-thrombin appears to be unique. The conversion of fibrinogen into fibrin by the serine protease enzyme thrombin is a major event in the final stages of blood coagulation. In the final stages of coagulation prothrombinase converts prothrombin into thrombin. Fibrin is subsequently cross linked by factor XIII to form a blood clot. The primary inhibitor of thrombin in normal blood circulation is antithrombin III. The anticoagulatant activity of hirudin is derived from its ability to inhibit the pro-coagulant activity of thrombin （similar to antithrombin III activity）. Hirudin is the strongest natural inhibitor of thrombin. Hirudin binds to ａnd inhibits only the activity of thrombin forms with a specific activity on fibrinogen contrasting to antithrombin III activity. Therefore, hirudin has a thrombolytic activity since it prevents or dissolves the formation of clots ａnd thrombi. Hirudin also has therapeutic significance in blood coagulation disorders, in the treatment of skin hematomas ａnd of superficial varicose veins. Hirudin does not hinder with the biological activity of other serum proteins ａnd can also act on complexed thrombin, thus having an advantage over more common anticoagulants ａnd thrombolytics like heparin for example. It is complicated to extract large quantities of hirudin from natural sources; therefore a method for producing ａnd purifying hirudin using recombinant biotechnology has been developed. Description Recombinant Hirudin is derived from yeast ａnd the polypeptide chain contains 65 amino acids ａnd its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule ａnd the absence of a sulfate group on the tyrosine at position 63.
The Recombinant Hirudin is purified by proprietary chromatographic techniques. Source Pichia Pastoris. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 ａnd 2% mannitol. Solubility It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Hirudin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Biological Activity The bilogical activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Hirudin/重组水蛭素
产品编号：PRO-362
产品规格：2μg/10μg/1mg
Introduction Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech unlike heparin. Hirudin acts directly on thrombin rather than through other clotting factors. The mechanism of Hirudin-thrombin appears to be unique. The conversion of fibrinogen into fibrin by the serine protease enzyme thrombin is a major event in the final stages of blood coagulation. In the final stages of coagulation prothrombinase converts prothrombin into thrombin. Fibrin is subsequently cross linked by factor XIII to form a blood clot. The primary inhibitor of thrombin in normal blood circulation is antithrombin III. The anticoagulatant activity of hirudin is derived from its ability to inhibit the pro-coagulant activity of thrombin （similar to antithrombin III activity）. Hirudin is the strongest natural inhibitor of thrombin. Hirudin binds to ａnd inhibits only the activity of thrombin forms with a specific activity on fibrinogen contrasting to antithrombin III activity. Therefore, hirudin has a thrombolytic activity since it prevents or dissolves the formation of clots ａnd thrombi. Hirudin also has therapeutic significance in blood coagulation disorders, in the treatment of skin hematomas ａnd of superficial varicose veins. Hirudin does not hinder with the biological activity of other serum proteins ａnd can also act on complexed thrombin, thus having an advantage over more common anticoagulants ａnd thrombolytics like heparin for example. It is complicated to extract large quantities of hirudin from natural sources; therefore a method for producing ａnd purifying hirudin using recombinant biotechnology has been developed. Description Recombinant Hirudin is derived from yeast ａnd the polypeptide chain contains 65 amino acids ａnd its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule ａnd the absence of a sulfate group on the tyrosine at position 63.
The Recombinant Hirudin is purified by proprietary chromatographic techniques. Source Pichia Pastoris. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 ａnd 2% mannitol. Solubility It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Hirudin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Biological Activity The bilogical activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Hirudin/重组水蛭素
产品编号：PRO-362
产品规格：2μg/10μg/1mg
Introduction Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech unlike heparin. Hirudin acts directly on thrombin rather than through other clotting factors. The mechanism of Hirudin-thrombin appears to be unique. The conversion of fibrinogen into fibrin by the serine protease enzyme thrombin is a major event in the final stages of blood coagulation. In the final stages of coagulation prothrombinase converts prothrombin into thrombin. Fibrin is subsequently cross linked by factor XIII to form a blood clot. The primary inhibitor of thrombin in normal blood circulation is antithrombin III. The anticoagulatant activity of hirudin is derived from its ability to inhibit the pro-coagulant activity of thrombin （similar to antithrombin III activity）. Hirudin is the strongest natural inhibitor of thrombin. Hirudin binds to ａnd inhibits only the activity of thrombin forms with a specific activity on fibrinogen contrasting to antithrombin III activity. Therefore, hirudin has a thrombolytic activity since it prevents or dissolves the formation of clots ａnd thrombi. Hirudin also has therapeutic significance in blood coagulation disorders, in the treatment of skin hematomas ａnd of superficial varicose veins. Hirudin does not hinder with the biological activity of other serum proteins ａnd can also act on complexed thrombin, thus having an advantage over more common anticoagulants ａnd thrombolytics like heparin for example. It is complicated to extract large quantities of hirudin from natural sources; therefore a method for producing ａnd purifying hirudin using recombinant biotechnology has been developed. Description Recombinant Hirudin is derived from yeast ａnd the polypeptide chain contains 65 amino acids ａnd its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule ａnd the absence of a sulfate group on the tyrosine at position 63.
The Recombinant Hirudin is purified by proprietary chromatographic techniques. Source Pichia Pastoris. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 ａnd 2% mannitol. Solubility It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Hirudin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Biological Activity The bilogical activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Hirudin/重组水蛭素
产品编号：PRO-362
产品规格：2μg/10μg/1mg
Introduction Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech unlike heparin. Hirudin acts directly on thrombin rather than through other clotting factors. The mechanism of Hirudin-thrombin appears to be unique. The conversion of fibrinogen into fibrin by the serine protease enzyme thrombin is a major event in the final stages of blood coagulation. In the final stages of coagulation prothrombinase converts prothrombin into thrombin. Fibrin is subsequently cross linked by factor XIII to form a blood clot. The primary inhibitor of thrombin in normal blood circulation is antithrombin III. The anticoagulatant activity of hirudin is derived from its ability to inhibit the pro-coagulant activity of thrombin （similar to antithrombin III activity）. Hirudin is the strongest natural inhibitor of thrombin. Hirudin binds to ａnd inhibits only the activity of thrombin forms with a specific activity on fibrinogen contrasting to antithrombin III activity. Therefore, hirudin has a thrombolytic activity since it prevents or dissolves the formation of clots ａnd thrombi. Hirudin also has therapeutic significance in blood coagulation disorders, in the treatment of skin hematomas ａnd of superficial varicose veins. Hirudin does not hinder with the biological activity of other serum proteins ａnd can also act on complexed thrombin, thus having an advantage over more common anticoagulants ａnd thrombolytics like heparin for example. It is complicated to extract large quantities of hirudin from natural sources; therefore a method for producing ａnd purifying hirudin using recombinant biotechnology has been developed. Description Recombinant Hirudin is derived from yeast ａnd the polypeptide chain contains 65 amino acids ａnd its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule ａnd the absence of a sulfate group on the tyrosine at position 63.
The Recombinant Hirudin is purified by proprietary chromatographic techniques. Source Pichia Pastoris. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 ａnd 2% mannitol. Solubility It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Hirudin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Biological Activity The bilogical activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Hirudin/重组水蛭素
产品编号：PRO-362
产品规格：2μg/10μg/1mg
Introduction Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech unlike heparin. Hirudin acts directly on thrombin rather than through other clotting factors. The mechanism of Hirudin-thrombin appears to be unique. The conversion of fibrinogen into fibrin by the serine protease enzyme thrombin is a major event in the final stages of blood coagulation. In the final stages of coagulation prothrombinase converts prothrombin into thrombin. Fibrin is subsequently cross linked by factor XIII to form a blood clot. The primary inhibitor of thrombin in normal blood circulation is antithrombin III. The anticoagulatant activity of hirudin is derived from its ability to inhibit the pro-coagulant activity of thrombin （similar to antithrombin III activity）. Hirudin is the strongest natural inhibitor of thrombin. Hirudin binds to ａnd inhibits only the activity of thrombin forms with a specific activity on fibrinogen contrasting to antithrombin III activity. Therefore, hirudin has a thrombolytic activity since it prevents or dissolves the formation of clots ａnd thrombi. Hirudin also has therapeutic significance in blood coagulation disorders, in the treatment of skin hematomas ａnd of superficial varicose veins. Hirudin does not hinder with the biological activity of other serum proteins ａnd can also act on complexed thrombin, thus having an advantage over more common anticoagulants ａnd thrombolytics like heparin for example. It is complicated to extract large quantities of hirudin from natural sources; therefore a method for producing ａnd purifying hirudin using recombinant biotechnology has been developed. Description Recombinant Hirudin is derived from yeast ａnd the polypeptide chain contains 65 amino acids ａnd its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule ａnd the absence of a sulfate group on the tyrosine at position 63.
The Recombinant Hirudin is purified by proprietary chromatographic techniques. Source Pichia Pastoris. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 ａnd 2% mannitol. Solubility It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Hirudin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Biological Activity The bilogical activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Hirudin/重组水蛭素
产品编号：PRO-362
产品规格：2μg/10μg/1mg
Introduction Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech unlike heparin. Hirudin acts directly on thrombin rather than through other clotting factors. The mechanism of Hirudin-thrombin appears to be unique. The conversion of fibrinogen into fibrin by the serine protease enzyme thrombin is a major event in the final stages of blood coagulation. In the final stages of coagulation prothrombinase converts prothrombin into thrombin. Fibrin is subsequently cross linked by factor XIII to form a blood clot. The primary inhibitor of thrombin in normal blood circulation is antithrombin III. The anticoagulatant activity of hirudin is derived from its ability to inhibit the pro-coagulant activity of thrombin （similar to antithrombin III activity）. Hirudin is the strongest natural inhibitor of thrombin. Hirudin binds to ａnd inhibits only the activity of thrombin forms with a specific activity on fibrinogen contrasting to antithrombin III activity. Therefore, hirudin has a thrombolytic activity since it prevents or dissolves the formation of clots ａnd thrombi. Hirudin also has therapeutic significance in blood coagulation disorders, in the treatment of skin hematomas ａnd of superficial varicose veins. Hirudin does not hinder with the biological activity of other serum proteins ａnd can also act on complexed thrombin, thus having an advantage over more common anticoagulants ａnd thrombolytics like heparin for example. It is complicated to extract large quantities of hirudin from natural sources; therefore a method for producing ａnd purifying hirudin using recombinant biotechnology has been developed. Description Recombinant Hirudin is derived from yeast ａnd the polypeptide chain contains 65 amino acids ａnd its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule ａnd the absence of a sulfate group on the tyrosine at position 63.
The Recombinant Hirudin is purified by proprietary chromatographic techniques. Source Pichia Pastoris. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 ａnd 2% mannitol. Solubility It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Hirudin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Biological Activity The bilogical activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Hirudin/重组水蛭素
产品编号：PRO-362
产品规格：2μg/10μg/1mg
Introduction Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech unlike heparin. Hirudin acts directly on thrombin rather than through other clotting factors. The mechanism of Hirudin-thrombin appears to be unique. The conversion of fibrinogen into fibrin by the serine protease enzyme thrombin is a major event in the final stages of blood coagulation. In the final stages of coagulation prothrombinase converts prothrombin into thrombin. Fibrin is subsequently cross linked by factor XIII to form a blood clot. The primary inhibitor of thrombin in normal blood circulation is antithrombin III. The anticoagulatant activity of hirudin is derived from its ability to inhibit the pro-coagulant activity of thrombin （similar to antithrombin III activity）. Hirudin is the strongest natural inhibitor of thrombin. Hirudin binds to ａnd inhibits only the activity of thrombin forms with a specific activity on fibrinogen contrasting to antithrombin III activity. Therefore, hirudin has a thrombolytic activity since it prevents or dissolves the formation of clots ａnd thrombi. Hirudin also has therapeutic significance in blood coagulation disorders, in the treatment of skin hematomas ａnd of superficial varicose veins. Hirudin does not hinder with the biological activity of other serum proteins ａnd can also act on complexed thrombin, thus having an advantage over more common anticoagulants ａnd thrombolytics like heparin for example. It is complicated to extract large quantities of hirudin from natural sources; therefore a method for producing ａnd purifying hirudin using recombinant biotechnology has been developed. Description Recombinant Hirudin is derived from yeast ａnd the polypeptide chain contains 65 amino acids ａnd its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule ａnd the absence of a sulfate group on the tyrosine at position 63.
The Recombinant Hirudin is purified by proprietary chromatographic techniques. Source Pichia Pastoris. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 ａnd 2% mannitol. Solubility It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Hirudin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Biological Activity The bilogical activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Hirudin/重组水蛭素
产品编号：PRO-362
产品规格：2μg/10μg/1mg
Introduction Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech unlike heparin. Hirudin acts directly on thrombin rather than through other clotting factors. The mechanism of Hirudin-thrombin appears to be unique. The conversion of fibrinogen into fibrin by the serine protease enzyme thrombin is a major event in the final stages of blood coagulation. In the final stages of coagulation prothrombinase converts prothrombin into thrombin. Fibrin is subsequently cross linked by factor XIII to form a blood clot. The primary inhibitor of thrombin in normal blood circulation is antithrombin III. The anticoagulatant activity of hirudin is derived from its ability to inhibit the pro-coagulant activity of thrombin （similar to antithrombin III activity）. Hirudin is the strongest natural inhibitor of thrombin. Hirudin binds to ａnd inhibits only the activity of thrombin forms with a specific activity on fibrinogen contrasting to antithrombin III activity. Therefore, hirudin has a thrombolytic activity since it prevents or dissolves the formation of clots ａnd thrombi. Hirudin also has therapeutic significance in blood coagulation disorders, in the treatment of skin hematomas ａnd of superficial varicose veins. Hirudin does not hinder with the biological activity of other serum proteins ａnd can also act on complexed thrombin, thus having an advantage over more common anticoagulants ａnd thrombolytics like heparin for example. It is complicated to extract large quantities of hirudin from natural sources; therefore a method for producing ａnd purifying hirudin using recombinant biotechnology has been developed. Description Recombinant Hirudin is derived from yeast ａnd the polypeptide chain contains 65 amino acids ａnd its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule ａnd the absence of a sulfate group on the tyrosine at position 63.
The Recombinant Hirudin is purified by proprietary chromatographic techniques. Source Pichia Pastoris. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 ａnd 2% mannitol. Solubility It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Hirudin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Biological Activity The bilogical activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Hirudin/重组水蛭素
产品编号：PRO-362
产品规格：2μg/10μg/1mg
Introduction Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech unlike heparin. Hirudin acts directly on thrombin rather than through other clotting factors. The mechanism of Hirudin-thrombin appears to be unique. The conversion of fibrinogen into fibrin by the serine protease enzyme thrombin is a major event in the final stages of blood coagulation. In the final stages of coagulation prothrombinase converts prothrombin into thrombin. Fibrin is subsequently cross linked by factor XIII to form a blood clot. The primary inhibitor of thrombin in normal blood circulation is antithrombin III. The anticoagulatant activity of hirudin is derived from its ability to inhibit the pro-coagulant activity of thrombin （similar to antithrombin III activity）. Hirudin is the strongest natural inhibitor of thrombin. Hirudin binds to ａnd inhibits only the activity of thrombin forms with a specific activity on fibrinogen contrasting to antithrombin III activity. Therefore, hirudin has a thrombolytic activity since it prevents or dissolves the formation of clots ａnd thrombi. Hirudin also has therapeutic significance in blood coagulation disorders, in the treatment of skin hematomas ａnd of superficial varicose veins. Hirudin does not hinder with the biological activity of other serum proteins ａnd can also act on complexed thrombin, thus having an advantage over more common anticoagulants ａnd thrombolytics like heparin for example. It is complicated to extract large quantities of hirudin from natural sources; therefore a method for producing ａnd purifying hirudin using recombinant biotechnology has been developed. Description Recombinant Hirudin is derived from yeast ａnd the polypeptide chain contains 65 amino acids ａnd its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule ａnd the absence of a sulfate group on the tyrosine at position 63.
The Recombinant Hirudin is purified by proprietary chromatographic techniques. Source Pichia Pastoris. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 ａnd 2% mannitol. Solubility It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Hirudin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Biological Activity The bilogical activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Hirudin/重组水蛭素
产品编号：PRO-362
产品规格：2μg/10μg/1mg
产品规格：2μg/10μg/1mg
| Introduction | Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech unlike heparin. Hirudin acts directly on thrombin rather than through other clotting factors. The mechanism of Hirudin-thrombin appears to be unique. The conversion of fibrinogen into fibrin by the serine protease enzyme thrombin is a major event in the final stages of blood coagulation. In the final stages of coagulation prothrombinase converts prothrombin into thrombin. Fibrin is subsequently cross linked by factor XIII to form a blood clot. The primary inhibitor of thrombin in normal blood circulation is antithrombin III. The anticoagulatant activity of hirudin is derived from its ability to inhibit the pro-coagulant activity of thrombin （similar to antithrombin III activity）. Hirudin is the strongest natural inhibitor of thrombin. Hirudin binds to ａnd inhibits only the activity of thrombin forms with a specific activity on fibrinogen contrasting to antithrombin III activity. Therefore, hirudin has a thrombolytic activity since it prevents or dissolves the formation of clots ａnd thrombi. Hirudin also has therapeutic significance in blood coagulation disorders, in the treatment of skin hematomas ａnd of superficial varicose veins. Hirudin does not hinder with the biological activity of other serum proteins ａnd can also act on complexed thrombin, thus having an advantage over more common anticoagulants ａnd thrombolytics like heparin for example. It is complicated to extract large quantities of hirudin from natural sources; therefore a method for producing ａnd purifying hirudin using recombinant biotechnology has been developed. |
| Description | Recombinant Hirudin is derived from yeast ａnd the polypeptide chain contains 65 amino acids ａnd its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule ａnd the absence of a sulfate group on the tyrosine at position 63. The Recombinant Hirudin is purified by proprietary chromatographic techniques. |
| Source | Pichia Pastoris. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | Each mg of protein was lyophilized from a sterile solution containing no 20mM PBS pH-7 ａnd 2% mannitol. |
| Solubility | It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Hirudin should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. |
| Purity | Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. |
| Biological Activity | The bilogical activity is measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein. |
| Usage | Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
