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IkappaB-epsilon (Phospho-Ser22) Antibody
点击次数：172发布时间：2012/8/23 14:44:12
更新日期：2024/10/16 15:59:07
所 在 地：美洲
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| Reactivity | Applications | MW. | Source |
| H, M, R | WB IHC IF ELISA | 38 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
| Product Name | IkappaB-epsilon （Phospho-Ser22） Antibody |
| Synonyms | I-kappa-B-epsilon; IKBE; IkappaBepsilon; NF-kappa-BIE; NF-kappaB inhibitor epsilon; NFKBIE |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IkappaB-epsilon around the phosphorylation site of Ser22. |
| Immunogen Region | Internal |
| Clonality | Polyclonal |
| Modification sites | H:S22 M:S22 R:S22 |
| Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 |
| Specificity | IkappaB-epsilon （Phospho-Ser22） Antibody detects endogenous levels of IkappaB-epsilon only when phosphorylated at serine22. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
| Physical Form | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Concentration | 1 mg/ml |
| Storage/Stability | Store at -20℃/1 year |
| NCBI Gene Symbol | NFKBIE | ||||||||
| Database Links |
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| Application images |  Immunofluorescence analysis of HeLa cells, using IkappaB-epsilon （Phospho-Ser22） Antibody. The picture on the right is treated with the synthesized peptide.  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IkappaB-epsilon （Phospho-Ser22） Antibody. The picture on the right is treated with the synthesized peptide.  Western blot analysis of extracts from Jurkat cells treated with TNF-a 20ng/ml 30＇, using IkappaB-epsilon （Phospho-Ser22） Antibody. The lane on the right is treated with the synthesized peptide. |
