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A0021 NF-kappaB p105/p50 (Phospho-Ser927) Antibody
点击次数:214发布时间:2012/8/23 14:57:49
更新日期:2024/10/16 15:59:07
所 在 地:美洲
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详细内容
Reactivity | Applications | MW. | Source |
H, M | WB IHC IF ELISA | 105 kDa | Rabbit |
Product Name | NF-kappaB p105/p50 (Phospho-Ser927) Antibody |
Synonyms | DNA-binding factor KBF1; EBP-1; NF-kappa-B1 p84/NF-kappa-B1 p98; NFKB1; NFkB-p50; Nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit |
Immunogen | The antiserum was produced against synthesized peptide derived from human NF-kappaB p105/p50 around the phosphorylation site of Ser927. |
Immunogen Region | C-terminal |
Clonality | Polyclonal |
Modification sites | H:S927 M:S930 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 |
Specificity | NF-kappaB p105/p50 (Phospho-Ser927) Antibody detects endogenous levels of NF-kappaB p105/p50 only when phosphorylated at serine927. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | NFKB1 | ||||||||
Database Links |
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Application images | Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using NF-kappaB p105/p50 (Phospho-Ser927) Antibody. The picture on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p105/p50 (Phospho-Ser927) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from HeLa cells treated with LPS 100ng/ml 30', using NF-kappaB p105/p50 (Phospho-Ser927) Antibody. The lane on the right is treated with the synthesized peptide. |