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A0025 Shc (Phospho-Tyr427) Antibody
点击次数：331发布时间：2012/8/23 15:03:46
更新日期：2024/10/16 15:59:07
所 在 地：美洲
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A0025 Shc （Phospho-Tyr427） Antibody
| Reactivity | Applications | MW. | Source |
| H, M, R | WB IHC ELISA | 62 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
| Product Name | Shc （Phospho-Tyr427） Antibody |
| Synonyms | SH2 domain protein C1; SHC, SHC-transforming protein 1; SHCA; Src homology 2 domain-containing-transforming protein C1 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Shc around the phosphorylation site of Tyr427. |
| Immunogen Region | Internal |
| Clonality | Polyclonal |
| Modification sites | H:Y427 M:Y423 R:Y313 |
| Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 |
| Specificity | Shc （Phospho-Tyr427） Antibody detects endogenous levels of Shc only when phosphorylated at tyrosine427. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
| Physical Form | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Concentration | 1 mg/ml |
| Storage/Stability | Store at -20℃/1 year |
| NCBI Gene Symbol | SHC1 | ||||||||
| Database Links |
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| Application images |  Immunohistochemistry analysis of paraffin-embedded human brain, using Shc （Phospho-Tyr427） Antibody. The picture on the right is treated with the synthesized peptide.  Western blot analysis of extracts from 293 cells treated with EGF 200ng/ml 5＇, using Shc （Phospho-Tyr427） Antibody. The lane on the right is treated with the synthesized peptide. |
