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A0031 Smad3 (Phospho-Ser425) Antibody
点击次数：112发布时间：2012/8/23 15:20:48
更新日期：2024/10/16 15:59:07
所 在 地：美洲
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A0031 Smad3 （Phospho-Ser425） Antibody
| Reactivity | Applications | MW. | Source |
| H, M, R | WB IHC ELISA | 48 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
| Product Name | Smad3 （Phospho-Ser425） Antibody |
| Synonyms | JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3; Mothers against decapentaplegic homolog 3; SMAD 3; Smad 3 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser425. |
| Immunogen Region | C-terminal |
| Clonality | Polyclonal |
| Modification sites | H:S425 M:S425 R:S425 |
| Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 |
| Specificity | Smad3 （Phospho-Ser425） Antibody detects endogenous levels of Smad3 only when phosphorylated at serine425. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
| Physical Form | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Concentration | 1 mg/ml |
| Storage/Stability | Store at -20℃/1 year |
| NCBI Gene Symbol | SMAD3 | ||||||||
| Database Links |
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| Application images |  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Smad3 （Phospho-Ser425） Antibody. The picture on the right is treated with the synthesized peptide.  Western blot analysis of extracts from NIH-3T3 cells treated with IGF-I 100ng/ml 10＇, using Smad3 （Phospho-Ser425） Antibody. The lane on the right is treated with the synthesized peptide. |
