| Product Name | 4E-BP1 （Phospho-Thr69） Antibody |
| Synonyms | 4EBP1; EIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Insulin-stimulated EIF-4E binding protein PHAS-I; P/OKCL.6; PHAS-1; PHAS-I |
| Immunogen | The antiserum was produced against synthesized peptide derived from mouse 4E-BP1 around the phosphorylation site of Thr69. |
| Immunogen Region | Internal |
| Clonality | Polyclonal |
| Modification sites | M:T69 R:T69 |
| Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 |
| Specificity | 4E-BP1 （Phospho-Thr69） Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine69. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
| Physical Form | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Concentration | 1 mg/ml |
| Storage/Stability | Store at -20℃/1 year |
