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A0608 Akt (Phospho-Ser246) Antibody
点击次数:112发布时间:2012/8/29 15:51:23
更新日期:2024/10/16 15:59:07
所 在 地:美洲
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A0608 Akt (Phospho-Ser246) Antibody
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC IF ELISA | 55 kDa | Rabbit |
Product Name | Akt (Phospho-Ser246) Antibody |
Synonyms | AKT; AKT1 kinase; C-AKT; kinase Akt1; PKB; PKB-alpha; Protein kinase B; RAC; RAC-alpha serine/threonine kinase; RAC-PK-alpha |
Immunogen | The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Ser246. |
Immunogen Region | Internal |
Clonality | Polyclonal |
Modification sites | H:S246 M:S246 R:S246 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 |
Specificity | Akt (Phospho-Ser246) Antibody detects endogenous levels of Akt only when phosphorylated at serine246. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | AKT1 | ||||||||
Database Links |
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Application images | Immunofluorescence analysis of NIH-3T3 cells, using Akt (Phospho-Ser246) Antibody. The picture on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human ovary, using Akt (Phospho-Ser246) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from HeLa cells treated with Etoposide 25uM 24h, using Akt (Phospho-Ser246) Antibody. The lane on the right is treated with the synthesized peptide. |
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