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A0610 Akt (Phospho-Thr72) Antibody

点击次数:114发布时间:2012/8/29 15:55:15

A0610     Akt (Phospho-Thr72) Antibody

更新日期:2024/10/16 15:59:07

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相关标签:A0610 Akt (Phospho-Thr72) Antibody 

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A0610     Akt (Phospho-Thr72) Antibody

Reactivity Applications MW. Source
H, M, R WB IHC ELISA 55 kDa Rabbit

Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.

Product Name Akt (Phospho-Thr72) Antibody
Synonyms AKT; AKT1 kinase; C-AKT; kinase Akt1; PKB; PKB-alpha; Protein kinase B; RAC; RAC-alpha serine/threonine kinase; RAC-PK-alpha
Immunogen The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Thr72.
Immunogen Region N-terminal
Clonality Polyclonal
Modification sites H:T72 M:T72 R:T72
Application dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity Akt (Phospho-Thr72) Antibody detects endogenous levels of Akt only when phosphorylated at threonine72.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration 1 mg/ml
Storage/Stability Store at -20℃/1 year

 

NCBI Gene Symbol AKT1
Database Links
Application images
Akt (Phospho-Thr72) Antibody for Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human ovary, using Akt (Phospho-Thr72) Antibody. The picture on the right is treated with the synthesized peptide.

Akt (Phospho-Thr72) Antibody for Western Blot

Western blot analysis of extracts from NIH-3T3 cells treated with TNF-a 20ng/ml 30', using Akt (Phospho-Thr72) Antibody. The lane on the right is treated with the synthesized peptide.

 

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